Genetic control of hematopoietic kinetics revealed by analyses of allophenic mice and stem cell suicide

Gary Van Zant; Paul W. Eldridge; Richard R. Behringer; Michael J. Dewey

doi:10.1016/0092-8674(83)90096-X

Genetic control of hematopoietic kinetics revealed by analyses of allophenic mice and stem cell suicide

Gary Van Zant, Paul W. Eldridge, Richard R. Behringer, Michael J. Dewey

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Abstract

The pluripotential hematopoietic stem cell is influenced by at least one gene that differs between DBA/2 and C3H/He, and C57BL/6 inbred mouse strains. This gene(s) manifests itself by its effect on susceptibility to killing of spleen colony forming cells (CFU-S) caused by hydroxyurea (HU). In strains DBA/2 and C3H/He 20% of the CFU-S population is normally in S phase whereas practically none from strain C57BL/6 are synthesizing DNA. On the other hand, in C57BL/6↔DBA/2 allophenic mice we observed that the proportion of DBA/2 erythrocytes was higher than the proportion of DBA/2 lymphocytes; the fraction of platelets and neutrophils with the DBA/2 genotype fell between the values for erythrocytes and lymphocytes. Control experiments using mice congenic at the Fv-2 locus confirm that in both situations we are examining effects of a gene(s) other than Fv-2. For the effect on the S phase fraction of CFU-S, we refer to the gene(s) as Stk (stem cell kinetics). We suggest that the observed skewing in composition among the various mature blood cell types in C57BL/6↔DBA/2 allophenic mice is caused by allelic variants of the Stk gene. Such variation would favor the formation of DBA/2 erythrocytes, platelets, and neutrophils over those of the C57BL/6 genotype.

Original language	English (US)
Pages (from-to)	639-645
Number of pages	7
Journal	Cell
Volume	35
Issue number	3 PART 2
DOIs	https://doi.org/10.1016/0092-8674(83)90096-X
State	Published - Dec 1983
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/0092-8674(83)90096-X

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@article{a620139a89ef49c4997f323fb9e2f3a7,

title = "Genetic control of hematopoietic kinetics revealed by analyses of allophenic mice and stem cell suicide",

abstract = "The pluripotential hematopoietic stem cell is influenced by at least one gene that differs between DBA/2 and C3H/He, and C57BL/6 inbred mouse strains. This gene(s) manifests itself by its effect on susceptibility to killing of spleen colony forming cells (CFU-S) caused by hydroxyurea (HU). In strains DBA/2 and C3H/He 20% of the CFU-S population is normally in S phase whereas practically none from strain C57BL/6 are synthesizing DNA. On the other hand, in C57BL/6↔DBA/2 allophenic mice we observed that the proportion of DBA/2 erythrocytes was higher than the proportion of DBA/2 lymphocytes; the fraction of platelets and neutrophils with the DBA/2 genotype fell between the values for erythrocytes and lymphocytes. Control experiments using mice congenic at the Fv-2 locus confirm that in both situations we are examining effects of a gene(s) other than Fv-2. For the effect on the S phase fraction of CFU-S, we refer to the gene(s) as Stk (stem cell kinetics). We suggest that the observed skewing in composition among the various mature blood cell types in C57BL/6↔DBA/2 allophenic mice is caused by allelic variants of the Stk gene. Such variation would favor the formation of DBA/2 erythrocytes, platelets, and neutrophils over those of the C57BL/6 genotype.",

author = "{Van Zant}, Gary and Eldridge, {Paul W.} and Behringer, {Richard R.} and Dewey, {Michael J.}",

note = "Funding Information: Thus work was partially supported by a Grant-In-Aid (82 968) from the Amerrcan Heart Assocratron, with funds contributed in part by the Mrssourr Affiliate, and by the Grace B. Reagan Memonal Grant for Cancer Research and grant #IN-107E, both from the Amencan Cancer Socrety. We are grateful to C. Glenn Fry for expert technical assrstance, S. Ttmmerman for help rn the preparation of the manuscript, and Drs. Clive Bunn and Donald Shreffler for critrcal reading of the manuscript and helpful discussions. We thank Dr. Eugene Goldwasser for the erythropoietin used in these studies.",

year = "1983",

month = dec,

doi = "10.1016/0092-8674(83)90096-X",

language = "English (US)",

volume = "35",

pages = "639--645",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "3 PART 2",

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TY - JOUR

T1 - Genetic control of hematopoietic kinetics revealed by analyses of allophenic mice and stem cell suicide

AU - Van Zant, Gary

AU - Eldridge, Paul W.

AU - Behringer, Richard R.

AU - Dewey, Michael J.

N1 - Funding Information: Thus work was partially supported by a Grant-In-Aid (82 968) from the Amerrcan Heart Assocratron, with funds contributed in part by the Mrssourr Affiliate, and by the Grace B. Reagan Memonal Grant for Cancer Research and grant #IN-107E, both from the Amencan Cancer Socrety. We are grateful to C. Glenn Fry for expert technical assrstance, S. Ttmmerman for help rn the preparation of the manuscript, and Drs. Clive Bunn and Donald Shreffler for critrcal reading of the manuscript and helpful discussions. We thank Dr. Eugene Goldwasser for the erythropoietin used in these studies.

PY - 1983/12

Y1 - 1983/12

N2 - The pluripotential hematopoietic stem cell is influenced by at least one gene that differs between DBA/2 and C3H/He, and C57BL/6 inbred mouse strains. This gene(s) manifests itself by its effect on susceptibility to killing of spleen colony forming cells (CFU-S) caused by hydroxyurea (HU). In strains DBA/2 and C3H/He 20% of the CFU-S population is normally in S phase whereas practically none from strain C57BL/6 are synthesizing DNA. On the other hand, in C57BL/6↔DBA/2 allophenic mice we observed that the proportion of DBA/2 erythrocytes was higher than the proportion of DBA/2 lymphocytes; the fraction of platelets and neutrophils with the DBA/2 genotype fell between the values for erythrocytes and lymphocytes. Control experiments using mice congenic at the Fv-2 locus confirm that in both situations we are examining effects of a gene(s) other than Fv-2. For the effect on the S phase fraction of CFU-S, we refer to the gene(s) as Stk (stem cell kinetics). We suggest that the observed skewing in composition among the various mature blood cell types in C57BL/6↔DBA/2 allophenic mice is caused by allelic variants of the Stk gene. Such variation would favor the formation of DBA/2 erythrocytes, platelets, and neutrophils over those of the C57BL/6 genotype.

AB - The pluripotential hematopoietic stem cell is influenced by at least one gene that differs between DBA/2 and C3H/He, and C57BL/6 inbred mouse strains. This gene(s) manifests itself by its effect on susceptibility to killing of spleen colony forming cells (CFU-S) caused by hydroxyurea (HU). In strains DBA/2 and C3H/He 20% of the CFU-S population is normally in S phase whereas practically none from strain C57BL/6 are synthesizing DNA. On the other hand, in C57BL/6↔DBA/2 allophenic mice we observed that the proportion of DBA/2 erythrocytes was higher than the proportion of DBA/2 lymphocytes; the fraction of platelets and neutrophils with the DBA/2 genotype fell between the values for erythrocytes and lymphocytes. Control experiments using mice congenic at the Fv-2 locus confirm that in both situations we are examining effects of a gene(s) other than Fv-2. For the effect on the S phase fraction of CFU-S, we refer to the gene(s) as Stk (stem cell kinetics). We suggest that the observed skewing in composition among the various mature blood cell types in C57BL/6↔DBA/2 allophenic mice is caused by allelic variants of the Stk gene. Such variation would favor the formation of DBA/2 erythrocytes, platelets, and neutrophils over those of the C57BL/6 genotype.

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U2 - 10.1016/0092-8674(83)90096-X

DO - 10.1016/0092-8674(83)90096-X

M3 - Article

C2 - 6652681

AN - SCOPUS:0021073667

SN - 0092-8674

VL - 35

SP - 639

EP - 645

JO - Cell

JF - Cell

IS - 3 PART 2

ER -

Genetic control of hematopoietic kinetics revealed by analyses of allophenic mice and stem cell suicide

Abstract

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