Genome analysis of Peromyscus (Rodentia, Cricetidae) - VII. Localization of satellite DNA sequences and cytoplasmic poly(A) RNA sequences of P. eremicus on metaphase chromosomes

Marion Watt Hazen; M. Tien Kuo; Frances E. Arrighi

doi:10.1007/BF00327053

Genome analysis of Peromyscus (Rodentia, Cricetidae) - VII. Localization of satellite DNA sequences and cytoplasmic poly(A) RNA sequences of P. eremicus on metaphase chromosomes

Marion Watt Hazen, M. Tien Kuo, Frances E. Arrighi

Translational Molecular Pathology

Research output: Contribution to journal › Article › peer-review

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Abstract

A satellite DNA fraction from P. eremicus, having a buoyant density of 1.705g/ml in neutral CsCl density gradients, was isolated. In situ hybridization experiments, using ³H-RNA complementary to this DNA fraction indicated that the short (heterochromatic) arms of most of the autosomes contained this sequence. Conversely, in situ hybridization using ³H-complementary DNA (cDNA) synthesized from the cytoplasmic poly (A) RNA of P. eremicus (comprising a substantial fraction of total messenger RNA) showed that the number of silver grains in the long arms (euchromatin) was significantly higher than that in the short arms. The X chromosomes showed a distinct localization pattern of both sequences.

Original language	English (US)
Pages (from-to)	133-142
Number of pages	10
Journal	Chromosoma
Volume	64
Issue number	2
DOIs	https://doi.org/10.1007/BF00327053
State	Published - Jun 1977

ASJC Scopus subject areas

Genetics
Genetics(clinical)

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title = "Genome analysis of Peromyscus (Rodentia, Cricetidae) - VII. Localization of satellite DNA sequences and cytoplasmic poly(A) RNA sequences of P. eremicus on metaphase chromosomes",

abstract = "A satellite DNA fraction from P. eremicus, having a buoyant density of 1.705g/ml in neutral CsCl density gradients, was isolated. In situ hybridization experiments, using 3H-RNA complementary to this DNA fraction indicated that the short (heterochromatic) arms of most of the autosomes contained this sequence. Conversely, in situ hybridization using 3H-complementary DNA (cDNA) synthesized from the cytoplasmic poly (A) RNA of P. eremicus (comprising a substantial fraction of total messenger RNA) showed that the number of silver grains in the long arms (euchromatin) was significantly higher than that in the short arms. The X chromosomes showed a distinct localization pattern of both sequences.",

author = "{Watt Hazen}, Marion and {Tien Kuo}, M. and Arrighi, {Frances E.}",

year = "1977",

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T1 - Genome analysis of Peromyscus (Rodentia, Cricetidae) - VII. Localization of satellite DNA sequences and cytoplasmic poly(A) RNA sequences of P. eremicus on metaphase chromosomes

AU - Watt Hazen, Marion

AU - Tien Kuo, M.

AU - Arrighi, Frances E.

PY - 1977/6

Y1 - 1977/6

N2 - A satellite DNA fraction from P. eremicus, having a buoyant density of 1.705g/ml in neutral CsCl density gradients, was isolated. In situ hybridization experiments, using 3H-RNA complementary to this DNA fraction indicated that the short (heterochromatic) arms of most of the autosomes contained this sequence. Conversely, in situ hybridization using 3H-complementary DNA (cDNA) synthesized from the cytoplasmic poly (A) RNA of P. eremicus (comprising a substantial fraction of total messenger RNA) showed that the number of silver grains in the long arms (euchromatin) was significantly higher than that in the short arms. The X chromosomes showed a distinct localization pattern of both sequences.

AB - A satellite DNA fraction from P. eremicus, having a buoyant density of 1.705g/ml in neutral CsCl density gradients, was isolated. In situ hybridization experiments, using 3H-RNA complementary to this DNA fraction indicated that the short (heterochromatic) arms of most of the autosomes contained this sequence. Conversely, in situ hybridization using 3H-complementary DNA (cDNA) synthesized from the cytoplasmic poly (A) RNA of P. eremicus (comprising a substantial fraction of total messenger RNA) showed that the number of silver grains in the long arms (euchromatin) was significantly higher than that in the short arms. The X chromosomes showed a distinct localization pattern of both sequences.

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Genome analysis of Peromyscus (Rodentia, Cricetidae) - VII. Localization of satellite DNA sequences and cytoplasmic poly(A) RNA sequences of P. eremicus on metaphase chromosomes

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