Genome-Wide CRISPR-Cas9 Screens Expose Genetic Vulnerabilities and Mechanisms of Temozolomide Sensitivity in Glioblastoma Stem Cells

Graham MacLeod; Danielle A. Bozek; Nishani Rajakulendran; Vernon Monteiro; Moloud Ahmadi; Zachary Steinhart; Michelle M. Kushida; Helen Yu; Fiona J. Coutinho; Florence M.G. Cavalli; Ian Restall; Xiaoguang Hao; Traver Hart; H. Artee Luchman; Samuel Weiss; Peter B. Dirks; Stephane Angers

doi:10.1016/j.celrep.2019.03.047

Genome-Wide CRISPR-Cas9 Screens Expose Genetic Vulnerabilities and Mechanisms of Temozolomide Sensitivity in Glioblastoma Stem Cells

Graham MacLeod, Danielle A. Bozek, Nishani Rajakulendran, Vernon Monteiro, Moloud Ahmadi, Zachary Steinhart, Michelle M. Kushida, Helen Yu, Fiona J. Coutinho, Florence M.G. Cavalli, Ian Restall, Xiaoguang Hao, Traver Hart, H. Artee Luchman, Samuel Weiss, Peter B. Dirks, Stephane Angers

Bioinformatics & Computational Biology

Research output: Contribution to journal › Article › peer-review

121 Scopus citations

Abstract

MacLeod et al. describe genome-wide CRISPR-Cas9 screens identifying genetic vulnerabilities across a panel of patient-derived glioblastoma stem cell cultures. Regulators of stemness (SOX2, SOX9, DOT1L, and SOCS3) and stress response (ufmylation and ERAD pathways) govern the growth of glioblastoma stem cells. Chemogenomic screens using temozolomide identify modulators of sensitivity to chemotherapy.

Original language	English (US)
Pages (from-to)	971-986.e9
Journal	Cell Reports
Volume	27
Issue number	3
DOIs	https://doi.org/10.1016/j.celrep.2019.03.047
State	Published - Apr 16 2019

Keywords

CRISPR-Cas9
fitness genes
functional genomics
glioblastoma
glioblastoma stem cells

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

MD Anderson CCSG core facilities

Bioinformatics Shared Resource

Access to Document

10.1016/j.celrep.2019.03.047

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MacLeod, G., Bozek, D. A., Rajakulendran, N., Monteiro, V., Ahmadi, M., Steinhart, Z., Kushida, M. M., Yu, H., Coutinho, F. J., Cavalli, F. M. G., Restall, I., Hao, X., Hart, T., Luchman, H. A., Weiss, S., Dirks, P. B., & Angers, S. (2019). Genome-Wide CRISPR-Cas9 Screens Expose Genetic Vulnerabilities and Mechanisms of Temozolomide Sensitivity in Glioblastoma Stem Cells. Cell Reports, 27(3), 971-986.e9. https://doi.org/10.1016/j.celrep.2019.03.047

MacLeod, G, Bozek, DA, Rajakulendran, N, Monteiro, V, Ahmadi, M, Steinhart, Z, Kushida, MM, Yu, H, Coutinho, FJ, Cavalli, FMG, Restall, I, Hao, X, Hart, T, Luchman, HA, Weiss, S, Dirks, PB & Angers, S 2019, 'Genome-Wide CRISPR-Cas9 Screens Expose Genetic Vulnerabilities and Mechanisms of Temozolomide Sensitivity in Glioblastoma Stem Cells', Cell Reports, vol. 27, no. 3, pp. 971-986.e9. https://doi.org/10.1016/j.celrep.2019.03.047

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abstract = "MacLeod et al. describe genome-wide CRISPR-Cas9 screens identifying genetic vulnerabilities across a panel of patient-derived glioblastoma stem cell cultures. Regulators of stemness (SOX2, SOX9, DOT1L, and SOCS3) and stress response (ufmylation and ERAD pathways) govern the growth of glioblastoma stem cells. Chemogenomic screens using temozolomide identify modulators of sensitivity to chemotherapy.",

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author = "Graham MacLeod and Bozek, {Danielle A.} and Nishani Rajakulendran and Vernon Monteiro and Moloud Ahmadi and Zachary Steinhart and Kushida, {Michelle M.} and Helen Yu and Coutinho, {Fiona J.} and Cavalli, {Florence M.G.} and Ian Restall and Xiaoguang Hao and Traver Hart and Luchman, {H. Artee} and Samuel Weiss and Dirks, {Peter B.} and Stephane Angers",

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AU - Monteiro, Vernon

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Genome-Wide CRISPR-Cas9 Screens Expose Genetic Vulnerabilities and Mechanisms of Temozolomide Sensitivity in Glioblastoma Stem Cells

Abstract

Keywords

ASJC Scopus subject areas

MD Anderson CCSG core facilities

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