Gold Nanoparticles for Monitoring of Mesenchymal Stem-Cell-Loaded Bioresorbable Polymeric Wraps for Arteriovenous Fistula Maturation

Allan John R. Barcena, Joy Vanessa D. Perez, Jossana A. Damasco, Marvin R. Bernardino, Erin Marie D. San Valentin, Carleigh Klusman, Benjamin Martin, Andrea Cortes, Gino Martin Canlas, Huckie C. Del Mundo, Francisco M. Heralde, Rony Avritscher, Natalie Fowlkes, Richard R. Bouchard, Jizhong Cheng, Steven Y. Huang, Marites P. Melancon

Research output: Contribution to journalArticlepeer-review

Abstract

Mesenchymal stem cell (MSC)-seeded polymeric perivascular wraps have been shown to enhance arteriovenous fistula (AVF) maturation. However, the wraps’ radiolucency makes their placement and integrity difficult to monitor. Through electrospinning, we infused gold nanoparticles (AuNPs) into polycaprolactone (PCL) wraps to improve their radiopacity and tested whether infusion affects the previously reported beneficial effects of the wraps on the AVF’s outflow vein. Sprague Dawley rat MSCs were seeded on the surface of the wraps. We then compared the effects of five AVF treatments—no perivascular wrap (i.e., control), PCL wrap, PCL + MSC wrap, PCL-Au wrap, and PCL-Au + MSC wrap—on AVF maturation in a Sprague Dawley rat model of chronic kidney disease (n = 3 per group). Via micro-CT, AuNP-infused wraps demonstrated a significantly higher radiopacity compared to that of the wraps without AuNPs. Wraps with and without AuNPs equally reduced vascular stenoses, as seen via ultrasonography and histomorphometry. In the immunofluorescence analysis, representative MSC-seeded wraps demonstrated reduced neointimal staining for markers of infiltration with smooth muscle cells (α-SMA), inflammatory cells (CD45), and fibroblasts (vimentin) compared to that of the control and wraps without MSCs. In conclusion, AuNP infusion allows in vivo monitoring via micro-CT of MSC-seeded polymeric wraps over time, without compromising the benefits of the wrap for AVF maturation.

Original languageEnglish (US)
Article number11754
JournalInternational journal of molecular sciences
Volume24
Issue number14
DOIs
StatePublished - Jul 2023

Keywords

  • arteriovenous fistula
  • computed tomography
  • end-stage renal disease
  • gold nanoparticles
  • mesenchymal stem cells
  • neointimal hyperplasia
  • polymer
  • ultrasonography

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

MD Anderson CCSG core facilities

  • Research Animal Support Facility

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