TY - JOUR
T1 - goosecoid is not an essential component of the mouse gastrula organizer but is required for craniofacial and rib development
AU - Rivera-Pérez, Jaime A.
AU - Mallo, Moisés
AU - Gendron-Maguire, Maureen
AU - Gridley, Thomas
AU - Behringer, Richard R.
PY - 1995/9
Y1 - 1995/9
N2 - goosecoid (gsc) is an evolutionarily conserved homeobox gene expressed in the gastrula organizer region of a variety of vertebrate embryos, including zebrafish, Xenopus, chicken and mouse. To understand the role of gsc during mouse embryogenesis, we generated gsc-null mice by gene targeting in embryonic stem cells. Surprisingly, gsc-null embryos gastrulated and formed the primary body axes; gsc-null mice were born alive but died soon after birth with numerous craniofacial defects. In addition, rib fusions and sternum abnormalities were detected that varied depending upon the genetic background. Transplantation experiments suggest that the ovary does not provide gsc function to rescue gastrulation defects. These results demonstrate that gsc is not essential for organizer activity in the mouse but is required later during embryogenesis for craniofacial and rib cage development.
AB - goosecoid (gsc) is an evolutionarily conserved homeobox gene expressed in the gastrula organizer region of a variety of vertebrate embryos, including zebrafish, Xenopus, chicken and mouse. To understand the role of gsc during mouse embryogenesis, we generated gsc-null mice by gene targeting in embryonic stem cells. Surprisingly, gsc-null embryos gastrulated and formed the primary body axes; gsc-null mice were born alive but died soon after birth with numerous craniofacial defects. In addition, rib fusions and sternum abnormalities were detected that varied depending upon the genetic background. Transplantation experiments suggest that the ovary does not provide gsc function to rescue gastrulation defects. These results demonstrate that gsc is not essential for organizer activity in the mouse but is required later during embryogenesis for craniofacial and rib cage development.
KW - Craniofacial development
KW - Gastrulation
KW - Mouse
KW - Skeleton
KW - goosecoid
UR - http://www.scopus.com/inward/record.url?scp=0029124998&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029124998&partnerID=8YFLogxK
M3 - Article
C2 - 7555726
AN - SCOPUS:0029124998
SN - 0950-1991
VL - 121
SP - 3005
EP - 3012
JO - Development
JF - Development
IS - 9
ER -