Abstract
Growth inhibitory activity of recombinant α and β interferon on two human glioma cell lines, EFC-2 and KE cells, was determined by two different growth assays. Recombinant β interferon showed β slight growth inhibitory effect on EFC-2 cells at day 3, and maximum inhibition was seen on day 6 with an ID50 of 50 U/ml. Recombinant α interferon showed no significant growth inhibition at any concentration. KE cells were resistant to both recombinant α and β interferon. The growth inhibitory activity of recombinant β interferon on EFC-2 cells was not blocked by recombinant α interferon, although recombinant α and β interferons shared same receptors on EFC-2 cells. Addition of DFMO (α-difluoromethylornithine) to interferon in the media showed additive effect rather than synergistic effect in growth inhibition of glioma cells. Out of 7 glioma cell lines tested, 4 showed heterogeneous sensitivity to recombinant β interferon, and all were resistant to recombinant α interferon. These results suggest β differential sensitivity of EFC-2 cells to recombinant β interferon, as well as a heterogeneous sensitivity to recombinant β interferon among different glioma cell lines.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 323-330 |
| Number of pages | 8 |
| Journal | Journal of neuro-oncology |
| Volume | 5 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 1987 |
Keywords
- glioma cells
- in vitro
- recombinant interferon
ASJC Scopus subject areas
- Oncology
- Neurology
- Clinical Neurology
- Cancer Research