TY - JOUR
T1 - Heterogeneity of isozyme expression in tumor cells does not correlate with metastatic potential
AU - Aukerman, Sharon Lea
AU - Siciliano, Michael J.
AU - Fidler, Isaiah J.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1986/7
Y1 - 1986/7
N2 - The major purpose of these studies was to determine whether the expression of isozymes by tumor cells was heterogeneous among tumor cell subpopulations within a neoplasm and whether expression of one or another isozyme correlated with metastatic potential of tumor cells. The expression levels of 40 isozymes were determined in 56 cell lines, many of them clonal, from nine different murine and human tumors. The enzymes chosen for study are involved in nucleotide, carbohydrate and pentose phosphate metabolism, and as such are indicators of the general metabolic and differentiational status of the cell. The tumors studied included two murine and two human malignant melanomas, four murine fibrosarcomas, and one human prostatic adenocarcinoma. The lines isolated from these tumors consisted of cells that are tumorigenic non-metastatic, tumorigenic low metastatic and tumorigenic highly metastatic. Clonally derived cell lines from a given tumor differed in their expression of a number of different isozymes, including adenosine deaminase, creatine phosphokinase-B and lactate dehydrogenase. Different patterns of isozyme expression were observed among different tumor types as well as between tumors of the same type; however, there were no differences in isozyme expression for any enzyme tested that correlated with metastatic ability of tumor cells.
AB - The major purpose of these studies was to determine whether the expression of isozymes by tumor cells was heterogeneous among tumor cell subpopulations within a neoplasm and whether expression of one or another isozyme correlated with metastatic potential of tumor cells. The expression levels of 40 isozymes were determined in 56 cell lines, many of them clonal, from nine different murine and human tumors. The enzymes chosen for study are involved in nucleotide, carbohydrate and pentose phosphate metabolism, and as such are indicators of the general metabolic and differentiational status of the cell. The tumors studied included two murine and two human malignant melanomas, four murine fibrosarcomas, and one human prostatic adenocarcinoma. The lines isolated from these tumors consisted of cells that are tumorigenic non-metastatic, tumorigenic low metastatic and tumorigenic highly metastatic. Clonally derived cell lines from a given tumor differed in their expression of a number of different isozymes, including adenosine deaminase, creatine phosphokinase-B and lactate dehydrogenase. Different patterns of isozyme expression were observed among different tumor types as well as between tumors of the same type; however, there were no differences in isozyme expression for any enzyme tested that correlated with metastatic ability of tumor cells.
UR - http://www.scopus.com/inward/record.url?scp=0022447695&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022447695&partnerID=8YFLogxK
U2 - 10.1007/BF00117931
DO - 10.1007/BF00117931
M3 - Article
C2 - 3742891
AN - SCOPUS:0022447695
SN - 0262-0898
VL - 4
SP - 177
EP - 189
JO - Clinical & Experimental Metastasis
JF - Clinical & Experimental Metastasis
IS - 3
ER -