TY - JOUR
T1 - Heterogeneity of non-Hodgkin's lymphoma probed by nucleic acid cytometry
AU - Srigley, J.
AU - Barlogie, B.
AU - Butler, J. J.
AU - Osborne, B.
AU - Blick, M.
AU - Johnston, D.
AU - Kantarjian, H.
AU - Reuben, James M
AU - Batsakis, J.
AU - Freireich, Emil J
PY - 1985
Y1 - 1985
N2 - Flow cytometric analyses of cellular DNA, RNA, and double-stranded RNA content were performed on lymph nodes and extranodal tissue from 177 patients with non-Hodgkin's lymphoma. With increasing histologic grade, a higher incidence of aneuploidy, higher proliferative activity, and higher total and double-stranded RNA content were found. Despite considerable cytometric heterogeneity within histologic grades and morphologic subdivisions, conformity between cytometric and morphologic classifications was observed in 85% of cases. Among intermediate-grade and high-grade lymphomas, increased proliferative activity and diploidy were associated with more frequent responses to treatment. Thus, nucleic acid-derived parameters relate to morphologic subtypes and permit an objective approach to lymphoma classification based on ploidy, proliferation, and RNA characteristics that also had prognostic implications.
AB - Flow cytometric analyses of cellular DNA, RNA, and double-stranded RNA content were performed on lymph nodes and extranodal tissue from 177 patients with non-Hodgkin's lymphoma. With increasing histologic grade, a higher incidence of aneuploidy, higher proliferative activity, and higher total and double-stranded RNA content were found. Despite considerable cytometric heterogeneity within histologic grades and morphologic subdivisions, conformity between cytometric and morphologic classifications was observed in 85% of cases. Among intermediate-grade and high-grade lymphomas, increased proliferative activity and diploidy were associated with more frequent responses to treatment. Thus, nucleic acid-derived parameters relate to morphologic subtypes and permit an objective approach to lymphoma classification based on ploidy, proliferation, and RNA characteristics that also had prognostic implications.
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U2 - 10.1182/blood.v65.5.1090.bloodjournal6551090
DO - 10.1182/blood.v65.5.1090.bloodjournal6551090
M3 - Article
C2 - 3995168
AN - SCOPUS:0022000529
SN - 0006-4971
VL - 65
SP - 1090
EP - 1096
JO - Blood
JF - Blood
IS - 5
ER -