TY - JOUR
T1 - Hexokinase isozymes of normal human subcutaneous adipose tissue
AU - Lavis, Victor R.
N1 - Funding Information:
From the Department of’ Medicine, University of TexasM edicalSchool. Receivedfor publication January 27, 1978. Supported by Grant AM 02456 from the National Institutes of Health (NIAMDD]. search Grant from the Juvenile Diabetes Foundation. Address reprint requests io Victor R. Lavis, Department of Medicine. l_‘ni\‘ersity of Te.yas Medical School. P.O. Box 20708. Houston, Te.uas 77025. c 1978 hy Grune & Stratton, inc. 0026 -O495/7~/27O9-OOllSOl,~~J~
PY - 1978/9
Y1 - 1978/9
N2 - The isozymes of hexokinase in surgical specimens of human subcutaneous adipose tissue were separated by elution from DEAE-cellulose with linear KCl gradients at pH 7.4. Two peaks of activity were found: Peak 1 eluted at 0.05M KCl, and Peak 2 at 0.19M KCl. Michaelis constants (Km) for glucose were: Peak 1, 6.5 × 10-5M; Peak 2, 1.5 × 10-4M. Peak 2 was more susceptible than Peak 1 to inactivation by trypsin, 0.1 mg/ml, and was protected by 0.1M glucose. Both peaks were protected from heat inactivation (45°) by 0.1M glucose. Peak 2 comprised 66 ± 5 percent of the total hexokinase activity. No activity with the characteristics of hexokinase III was detected in human fat. In all these characteristics, the isozymes of human adipose tissue closely resemble hexokinases I and II from rats.
AB - The isozymes of hexokinase in surgical specimens of human subcutaneous adipose tissue were separated by elution from DEAE-cellulose with linear KCl gradients at pH 7.4. Two peaks of activity were found: Peak 1 eluted at 0.05M KCl, and Peak 2 at 0.19M KCl. Michaelis constants (Km) for glucose were: Peak 1, 6.5 × 10-5M; Peak 2, 1.5 × 10-4M. Peak 2 was more susceptible than Peak 1 to inactivation by trypsin, 0.1 mg/ml, and was protected by 0.1M glucose. Both peaks were protected from heat inactivation (45°) by 0.1M glucose. Peak 2 comprised 66 ± 5 percent of the total hexokinase activity. No activity with the characteristics of hexokinase III was detected in human fat. In all these characteristics, the isozymes of human adipose tissue closely resemble hexokinases I and II from rats.
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U2 - 10.1016/0026-0495(78)90156-7
DO - 10.1016/0026-0495(78)90156-7
M3 - Article
C2 - 682973
AN - SCOPUS:0018077631
SN - 0026-0495
VL - 27
SP - 1101
EP - 1108
JO - Metabolism
JF - Metabolism
IS - 9
ER -