TY - JOUR
T1 - High colony‐forming efficiency of primary human tumor cells cultured in the adhesive‐tumor‐cell culture system
T2 - Improvements with medium and serum alterations
AU - Baker, Fraser L.
AU - Ajanf, Jaffer
AU - Spitzer, Gary
AU - Tomasovic, Barbara J.
AU - Williams, Marcia
AU - Finders, Margot
AU - Brock, William A.
PY - 1988
Y1 - 1988
N2 - The colony‐forming efficiency (CFE) of primary human tumor cells cultured in the adhesive‐tumor‐cell culture system (ATCCS) using Ham's F12 (F12) or Eagle's minimum essential medium, alpha modification (alphaMEM) and culture medium supplemented with either swine, equine or bovine sera were compared. AlphaMEM supplemented with equine serum provided the highest CFE of the combinations. The CFE increase due to the change from F12 to alphaMEM was approximately 5‐fold, and the increase due to the change in serum from swine to equine was approximately 2‐fold. Cytokeratin staining showed that this increase was not due to fibroblast growth. The high‐average CFE with alphaMEM, approximately 3%, means that an inoculum of only 2 × 103 cells is needed to achieve formation of approximately 65 colonies in control cultures, thereby increasing the performance of this system when used in a chemosensitivity assay.
AB - The colony‐forming efficiency (CFE) of primary human tumor cells cultured in the adhesive‐tumor‐cell culture system (ATCCS) using Ham's F12 (F12) or Eagle's minimum essential medium, alpha modification (alphaMEM) and culture medium supplemented with either swine, equine or bovine sera were compared. AlphaMEM supplemented with equine serum provided the highest CFE of the combinations. The CFE increase due to the change from F12 to alphaMEM was approximately 5‐fold, and the increase due to the change in serum from swine to equine was approximately 2‐fold. Cytokeratin staining showed that this increase was not due to fibroblast growth. The high‐average CFE with alphaMEM, approximately 3%, means that an inoculum of only 2 × 103 cells is needed to achieve formation of approximately 65 colonies in control cultures, thereby increasing the performance of this system when used in a chemosensitivity assay.
KW - Adhesive‐tumor‐cell culture system
KW - Colony‐forming efficiency
KW - Primary human tumor cell cultures
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U2 - 10.1002/stem.5530060203
DO - 10.1002/stem.5530060203
M3 - Article
C2 - 3373036
AN - SCOPUS:0023886482
SN - 0737-1454
VL - 6
SP - 95
EP - 105
JO - The International Journal of Cell Cloning
JF - The International Journal of Cell Cloning
IS - 2
ER -