Abstract
The human α1-globin gene was fused downstream of two erythroid-specific DNase I super-hypersensitive sites that are normally located upstream of the human β-globin locus. This construct was injected into fertilized mouse eggs, and expression was analyzed in 16-day fetal livers and brains. All 11 fetuses that contained intact copies of the transgene expressed correctly initiated human α-globin mRNA in the erythroid fetal liver but not in brain. Levels of expression ranged from 4% to 337% of endogenous mouse β-globin mRNA. A human α-globin construct that did not contain super-hypersensitive sites was not expressed. These results demonstrate that human β-globin locus activation sequences can stimulate high levels of human α-globin gene expression in erythroid tissue of transgenic mice. The results also provide a foundation for experiments designed to coexpress human α- and β-globin genes in transgenic mice and suggest a feasible approach for production of a mouse model for human sickle cell disease.
Original language | English (US) |
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Pages (from-to) | 37-41 |
Number of pages | 5 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 86 |
Issue number | 1 |
DOIs | |
State | Published - 1989 |
Externally published | Yes |
ASJC Scopus subject areas
- General