High-performance liquid chromatographic method for the simultaneous measurement of floxuridine and fluorouracil in human body fluids

Joyce A. Smith-Rogers; William P. Tong; Mary E. Duafala; Maurie Markman; Joseph R. Bertino

doi:10.1016/0378-4347(91)80119-W

High-performance liquid chromatographic method for the simultaneous measurement of floxuridine and fluorouracil in human body fluids

Joyce A. Smith-Rogers, William P. Tong, Mary E. Duafala, Maurie Markman, Joseph R. Bertino

Cancer Systems Imaging

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11 Scopus citations

Abstract

A method for the simultaneous measurement of floxuridine (5-fluorodeoxyuridine) and fluorouracil in human plasma and peritoneal fluid has been developed. This method utilizes high-performance liquid chromatographic analysis with a Zorbax RX column (25 cm × 4.6 mm I.D.) plus a guard cartridge of the same material. The sensitivity limits for this assay are 0.25 μmol/l in a 20-μl sample. The detection limit at a signal-to-noise ratio of 3 is 2.5 nmol/l. This procedure has been used to quantitatively measure concentration versus time profiles of floxuridine and fluorouracil in plasma and peritoneal fluid of human patients after receiving intraperitoneal administration of floxuridine.

Original language	English (US)
Pages (from-to)	147-154
Number of pages	8
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	566
Issue number	1
DOIs	https://doi.org/10.1016/0378-4347(91)80119-W
State	Published - May 3 1991

ASJC Scopus subject areas

General Chemistry

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High-performance liquid chromatographic method for the simultaneous measurement of floxuridine and fluorouracil in human body fluids. / Smith-Rogers, Joyce A.; Tong, William P.; Duafala, Mary E. et al.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 566, No. 1, 03.05.1991, p. 147-154.

Research output: Contribution to journal › Article › peer-review

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High-performance liquid chromatographic method for the simultaneous measurement of floxuridine and fluorouracil in human body fluids

Abstract

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