TY - JOUR
T1 - High-resolution fiber optic microscopy with fluorescent contrast enhancement for the identification of axillary lymph node metastases in breast cancer
T2 - A pilot study
AU - Rosbach, Kelsey J.
AU - Shin, Dongsuk
AU - Muldoon, Timothy J.
AU - Quraishi, Mohammad A.
AU - Middleton, Lavinia P.
AU - Hunt, Kelly K.
AU - Meric-Bernstam, Funda
AU - Yu, Tse Kuan
AU - Richards-Kortum, Rebecca R.
AU - Yang, Wei
PY - 2010/10/1
Y1 - 2010/10/1
N2 - This prospective pilot study evaluates the potential of highresolution fiber optic microscopy (HRFM) to identify lymph node metastases in breast cancer patients. 43 lymph nodes were collected from 14 consenting breast cancer patients. Proflavine dye was topically applied to lymph nodes ex vivo to allow visualization of nuclei. 242 images were collected at 105 sites with confirmed histopathologic diagnosis. Quantitative statistical features were calculated from images, assessed with one-way ANOVA, and were used to develop a classification algorithm with the goal of objectively discriminating between normal and metastatic tissue. A classification algorithm using mean image intensity and skewness achieved sensitivity of 79% (27/34) and specificity of 77% (55/71). This study demonstrates the technical feasibility and diagnostic potential of HRFM with fluorescent contrast in the ex vivo evaluation of lymph nodes from breast cancer patients.
AB - This prospective pilot study evaluates the potential of highresolution fiber optic microscopy (HRFM) to identify lymph node metastases in breast cancer patients. 43 lymph nodes were collected from 14 consenting breast cancer patients. Proflavine dye was topically applied to lymph nodes ex vivo to allow visualization of nuclei. 242 images were collected at 105 sites with confirmed histopathologic diagnosis. Quantitative statistical features were calculated from images, assessed with one-way ANOVA, and were used to develop a classification algorithm with the goal of objectively discriminating between normal and metastatic tissue. A classification algorithm using mean image intensity and skewness achieved sensitivity of 79% (27/34) and specificity of 77% (55/71). This study demonstrates the technical feasibility and diagnostic potential of HRFM with fluorescent contrast in the ex vivo evaluation of lymph nodes from breast cancer patients.
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U2 - 10.1364/BOE.1.000911
DO - 10.1364/BOE.1.000911
M3 - Article
C2 - 21258518
AN - SCOPUS:84859354416
SN - 2156-7085
VL - 1
SP - 911
EP - 922
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 3
ER -