@inbook{7e278bf7081e477f99f5bbcdc245874d,
title = "High-resolution melt curve analysis in cancer mutation screen",
abstract = "High-resolution melt (HRM) curve analysis is a PCR-based assay that identifi es sequence alterations based on subtle variations in the melting curves of mutated versus wild-type DNA sequences. HRM analysis is a high-throughput, sensitive, and effi cient alternative to Sanger sequencing and is used to assess for mutations in clinically important genes involved in cancer diagnosis. The technique involves PCR amplifi cation of a target sequence in the presence of a fl uorescent double-stranded DNA (dsDNA) binding dye, melting of the fl uorescent amplicons, and subsequent interpretation of melt curve profi les.",
keywords = "Double-stranded DNA binding fluorescent dyes, High-resolution melt curve analysis, PCR",
author = "Meenakshi Mehrotra and Patel, {Keyur P.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
month = feb,
day = "1",
doi = "10.1007/978-1-4939-3360-0_7",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "63--69",
booktitle = "Methods in Molecular Biology",
}