@inbook{c6f3cde022c1494390cedded53a8e3a9,
title = "High-throughput immunofluorescence microscopy using yeast spheroplast cell-based microarrays",
abstract = "We have described a protocol for performing high-throughput immunofluorescence microscopy on microarrays of yeast cells. This approach employs immunostaining of spheroplasted yeast cells printed as high-density cell microarrays, followed by imaging using automated microscopy. A yeast spheroplast microarray can contain more than 5,000 printed spots, each containing cells from a given yeast strain, and is thus suitable for genome-wide screens focusing on single cell phenotypes, such as systematic localization or co-localization studies or genetic assays for genes affecting probed targets. We demonstrate the use of yeast spheroplast microarrays to probe microtubule and spindle defects across a collection of yeast strains harboring tetracycline-down-regulatable alleles of essential genes.",
keywords = "Cell microarrays, High-throughput microscopy, Immunofluorescence, Microtubule, Yeast",
author = "Wei Niu and {Traver Hart}, G. and Marcotte, {Edward M.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC 2011.",
year = "2011",
doi = "10.1007/978-1-61737-970-3_7",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "83--95",
booktitle = "Methods in Molecular Biology",
}