Highly robust stainless steel tips as microelectrospray emitters

Yasushi Ishihama; Hiroyuki Katayama; Naoki Asakawa; Yoshiya Oda

doi:10.1002/rcm.647

Highly robust stainless steel tips as microelectrospray emitters

Yasushi Ishihama, Hiroyuki Katayama, Naoki Asakawa, Yoshiya Oda

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Tapered stainless steel spray tips for sheathless microelectrospray ionization (μESI) have been developed. The fabrication procedure for the tapered stainless steel tips was optimized using an electropolishing technique followed by removal of the burr. Using the tip as the μESI emitter, a stable ESI spray was obtained at a flow rate of 20 nL/min. The sensitivity of the μESI system was almost two orders greater than that of the conventional ion spray system. The tip was highly stable, and was successfully used for over 1000 h. Moreover, these stainless steel tips were suitable for use with sheathless capillary electrophoresis/mass spectrometry (CE/MS) and capillary liquid chromatography/mass spectrometry (LC/MS) for routine analysis in proteomic and pharmaceutical applications.

Original language	English (US)
Pages (from-to)	913-918
Number of pages	6
Journal	Rapid Communications in Mass Spectrometry
Volume	16
Issue number	10
DOIs	https://doi.org/10.1002/rcm.647
State	Published - 2002
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Spectroscopy
Organic Chemistry

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abstract = "Tapered stainless steel spray tips for sheathless microelectrospray ionization (μESI) have been developed. The fabrication procedure for the tapered stainless steel tips was optimized using an electropolishing technique followed by removal of the burr. Using the tip as the μESI emitter, a stable ESI spray was obtained at a flow rate of 20 nL/min. The sensitivity of the μESI system was almost two orders greater than that of the conventional ion spray system. The tip was highly stable, and was successfully used for over 1000 h. Moreover, these stainless steel tips were suitable for use with sheathless capillary electrophoresis/mass spectrometry (CE/MS) and capillary liquid chromatography/mass spectrometry (LC/MS) for routine analysis in proteomic and pharmaceutical applications.",

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AU - Ishihama, Yasushi

AU - Katayama, Hiroyuki

AU - Asakawa, Naoki

AU - Oda, Yoshiya

PY - 2002

Y1 - 2002

N2 - Tapered stainless steel spray tips for sheathless microelectrospray ionization (μESI) have been developed. The fabrication procedure for the tapered stainless steel tips was optimized using an electropolishing technique followed by removal of the burr. Using the tip as the μESI emitter, a stable ESI spray was obtained at a flow rate of 20 nL/min. The sensitivity of the μESI system was almost two orders greater than that of the conventional ion spray system. The tip was highly stable, and was successfully used for over 1000 h. Moreover, these stainless steel tips were suitable for use with sheathless capillary electrophoresis/mass spectrometry (CE/MS) and capillary liquid chromatography/mass spectrometry (LC/MS) for routine analysis in proteomic and pharmaceutical applications.

AB - Tapered stainless steel spray tips for sheathless microelectrospray ionization (μESI) have been developed. The fabrication procedure for the tapered stainless steel tips was optimized using an electropolishing technique followed by removal of the burr. Using the tip as the μESI emitter, a stable ESI spray was obtained at a flow rate of 20 nL/min. The sensitivity of the μESI system was almost two orders greater than that of the conventional ion spray system. The tip was highly stable, and was successfully used for over 1000 h. Moreover, these stainless steel tips were suitable for use with sheathless capillary electrophoresis/mass spectrometry (CE/MS) and capillary liquid chromatography/mass spectrometry (LC/MS) for routine analysis in proteomic and pharmaceutical applications.

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Highly robust stainless steel tips as microelectrospray emitters

Abstract

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