HIV-1 genotypic resistance testing on low viral load specimens using the Abbott ViroSeq HIV-1 Genotyping System

Zhen Yang; Richard Morrison; Carl Oates; Juan Sarria; Janak Patel; Afsoon Habibi; Rana Seyedjafari; Peter Hu; Alan Lennon; Albert Li; Jianli Dong

doi:10.1309/LMVOVU1XRB9O3JZV

HIV-1 genotypic resistance testing on low viral load specimens using the Abbott ViroSeq HIV-1 Genotyping System

Zhen Yang, Richard Morrison, Carl Oates, Juan Sarria, Janak Patel, Afsoon Habibi, Rana Seyedjafari, Peter Hu, Alan Lennon, Albert Li, Jianli Dong

School of Health Professions

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Background: The Abbott ViroSeq HIV-1 Genotyping System requires a minimum viral load of 2,000 copies/mL. The aim of this study was to evaluate the performance on samples of a viral load <2,000 copies/mL. Method: We performed a retrospective analysis of 780 genotypic tests performed at the University of Texas Medical Branch (UTMB) Molecular Diagnostics Laboratory between April 2007 and January 2008. Results: A quarter of the samples (25.5%, 199/780) had HIV viremia <2,000 copies/mL. Of the 199 specimens with plasma HIV RNA <2,000 copies/mL, genotyping was completed in 111 of 118 (94%) samples with quantifiable HIV RNA, but only in 48 of 81 (59%) specimens with a viral load <75 copies/mL. Conclusion: Our data does not support the conclusion of some investigators that HIV-1 genotypic resistance testing is technically unreliable on low viral load specimens.

Original language	English (US)
Pages (from-to)	671-673
Number of pages	3
Journal	Laboratory Medicine
Volume	39
Issue number	11
DOIs	https://doi.org/10.1309/LMVOVU1XRB9O3JZV
State	Published - 2008

ASJC Scopus subject areas

Clinical Biochemistry
Biochemistry, medical

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title = "HIV-1 genotypic resistance testing on low viral load specimens using the Abbott ViroSeq HIV-1 Genotyping System",

abstract = "Background: The Abbott ViroSeq HIV-1 Genotyping System requires a minimum viral load of 2,000 copies/mL. The aim of this study was to evaluate the performance on samples of a viral load <2,000 copies/mL. Method: We performed a retrospective analysis of 780 genotypic tests performed at the University of Texas Medical Branch (UTMB) Molecular Diagnostics Laboratory between April 2007 and January 2008. Results: A quarter of the samples (25.5%, 199/780) had HIV viremia <2,000 copies/mL. Of the 199 specimens with plasma HIV RNA <2,000 copies/mL, genotyping was completed in 111 of 118 (94%) samples with quantifiable HIV RNA, but only in 48 of 81 (59%) specimens with a viral load <75 copies/mL. Conclusion: Our data does not support the conclusion of some investigators that HIV-1 genotypic resistance testing is technically unreliable on low viral load specimens.",

author = "Zhen Yang and Richard Morrison and Carl Oates and Juan Sarria and Janak Patel and Afsoon Habibi and Rana Seyedjafari and Peter Hu and Alan Lennon and Albert Li and Jianli Dong",

year = "2008",

doi = "10.1309/LMVOVU1XRB9O3JZV",

language = "English (US)",

volume = "39",

pages = "671--673",

journal = "Laboratory Medicine",

issn = "0007-5027",

publisher = "American Society of Clinical Pathologists",

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TY - JOUR

T1 - HIV-1 genotypic resistance testing on low viral load specimens using the Abbott ViroSeq HIV-1 Genotyping System

AU - Yang, Zhen

AU - Morrison, Richard

AU - Oates, Carl

AU - Sarria, Juan

AU - Patel, Janak

AU - Habibi, Afsoon

AU - Seyedjafari, Rana

AU - Hu, Peter

AU - Lennon, Alan

AU - Li, Albert

AU - Dong, Jianli

PY - 2008

Y1 - 2008

N2 - Background: The Abbott ViroSeq HIV-1 Genotyping System requires a minimum viral load of 2,000 copies/mL. The aim of this study was to evaluate the performance on samples of a viral load <2,000 copies/mL. Method: We performed a retrospective analysis of 780 genotypic tests performed at the University of Texas Medical Branch (UTMB) Molecular Diagnostics Laboratory between April 2007 and January 2008. Results: A quarter of the samples (25.5%, 199/780) had HIV viremia <2,000 copies/mL. Of the 199 specimens with plasma HIV RNA <2,000 copies/mL, genotyping was completed in 111 of 118 (94%) samples with quantifiable HIV RNA, but only in 48 of 81 (59%) specimens with a viral load <75 copies/mL. Conclusion: Our data does not support the conclusion of some investigators that HIV-1 genotypic resistance testing is technically unreliable on low viral load specimens.

AB - Background: The Abbott ViroSeq HIV-1 Genotyping System requires a minimum viral load of 2,000 copies/mL. The aim of this study was to evaluate the performance on samples of a viral load <2,000 copies/mL. Method: We performed a retrospective analysis of 780 genotypic tests performed at the University of Texas Medical Branch (UTMB) Molecular Diagnostics Laboratory between April 2007 and January 2008. Results: A quarter of the samples (25.5%, 199/780) had HIV viremia <2,000 copies/mL. Of the 199 specimens with plasma HIV RNA <2,000 copies/mL, genotyping was completed in 111 of 118 (94%) samples with quantifiable HIV RNA, but only in 48 of 81 (59%) specimens with a viral load <75 copies/mL. Conclusion: Our data does not support the conclusion of some investigators that HIV-1 genotypic resistance testing is technically unreliable on low viral load specimens.

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U2 - 10.1309/LMVOVU1XRB9O3JZV

DO - 10.1309/LMVOVU1XRB9O3JZV

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SN - 0007-5027

VL - 39

SP - 671

EP - 673

JO - Laboratory Medicine

JF - Laboratory Medicine

IS - 11

ER -

HIV-1 genotypic resistance testing on low viral load specimens using the Abbott ViroSeq HIV-1 Genotyping System

Abstract

ASJC Scopus subject areas

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