TY - JOUR
T1 - How Do We Use Multicolor Flow Cytometry to Detect Minimal Residual Disease in Acute Myeloid Leukemia?
AU - Xu, Jie
AU - Jorgensen, Jeffrey L.
AU - Wang, Sa A.
N1 - Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2017/12
Y1 - 2017/12
N2 - Multicolor flow cytometry (MFC), combined with molecular and cytogenetic studies, is the most common method for detecting minimal residual disease (MRD) in acute myeloid leukemia (AML). Studies have shown that a positive MFC MRD study after induction and/or consolidation, or before allogeneic hematopoietic stem cell transplantation, correlates with risk of relapse and inferior survival. However, there is little information on technical and analytical details. This article shares the authors’ experience using MFC for AML MRD detection, including antibody panel design, data analysis, and interpretation. It summarizes diagnostic pearls and pitfalls, and provides practical information for pathologists or hematologists.
AB - Multicolor flow cytometry (MFC), combined with molecular and cytogenetic studies, is the most common method for detecting minimal residual disease (MRD) in acute myeloid leukemia (AML). Studies have shown that a positive MFC MRD study after induction and/or consolidation, or before allogeneic hematopoietic stem cell transplantation, correlates with risk of relapse and inferior survival. However, there is little information on technical and analytical details. This article shares the authors’ experience using MFC for AML MRD detection, including antibody panel design, data analysis, and interpretation. It summarizes diagnostic pearls and pitfalls, and provides practical information for pathologists or hematologists.
KW - Acute myeloid leukemia
KW - Minimal residual disease
KW - Multicolor flow cytometry
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U2 - 10.1016/j.cll.2017.07.004
DO - 10.1016/j.cll.2017.07.004
M3 - Review article
C2 - 29128069
AN - SCOPUS:85030171106
SN - 0272-2712
VL - 37
SP - 787
EP - 802
JO - Clinics in Laboratory Medicine
JF - Clinics in Laboratory Medicine
IS - 4
ER -