Human- B and T-lymphocyte locomotion in response to casein, C5a, and f-Met-Leu-Phe

Human peripheral blood monocyte-depleted lymphocytes, T lymphocytes, and non-T lyphocytes were studied for their locomotor activity in response to several common chemotactic stimuli. The factors used to stimulate lymphocyte locomotion were casein, C5a, and f-Met-Leu-Phe. Chemotaxis (directional locomotion) as well as chemokinesis (nondirectional locomotion) in response to each factor were delineated. Monocyte-depleted lymphocyte locomotion was stimulated significantly by all of the above factors. Separation of lymphocytes into T cells and non-T cells indicated that T-lymphocyte locomotion was stimulated by casein and C5a but not by f-Met-Leu-Phe. Non-T lymphocytes were found to respond to C5a and f-Met-Leu-Phe but responded minimally to casein. Additional experiments indicated that casein and f-Met-Leu-Phe were chemokinetic for both monocyte-depleted lymphocytes and non-T lymphocytes, while C5a was chemotactic for both monocyte-depleted lymphocyte preparations and purified T cells.