TY - JOUR
T1 - Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family
AU - Koff, Andrew
AU - Cross, Fred
AU - Fisher, Alfred
AU - Schumacher, Jill
AU - Leguellec, Katherine
AU - Philippe, Michel
AU - Roberts, James M.
N1 - Funding Information:
We thank Linda Breeden and Steve Henikoff for help throughout the course of this work and Steve Reed, Danny Lew, Vjekoslav Dulic, and Steve Elledge for communication of results prior to publication. We would also like to thank numerous colleagues at the Hutchinson Center and at Rockefeller University for suggestions and comments on these experiments. This work was supported in part by a grant to J. M. R. from the NIH. J. M. R. and F. C. are Lucille P. Markey scholars in the biomedical sciences, and this work was supported in part by a grant from the Lucille P. Markey Charitable Trust.
PY - 1991/9/20
Y1 - 1991/9/20
N2 - A new human cyclin, named cyclin E, was isolated by complementation of a triple cln deletion in S. cerevisiae. Cyclin E showed genetic interactions with the CDC28 gene, suggesting that it functioned at START by interacting with the CDC28 protein. Two human genes were identified that could interact with cyclin E to perform START in yeast containing a cdc28 mutation. One was CDC2-HS, and the second was the human homolog of Xenopus CDK2. Cyclin E produced in E. coli bound and activated the CDC2 protein in extracts from human G1 cells, and antibodies against cyclin E immunoprecipitated a histone H1 kinase from HeLa cells. The interactions between cyclin E and CDC2, or CDK2, may be important at the G1 to S transition in human cells.
AB - A new human cyclin, named cyclin E, was isolated by complementation of a triple cln deletion in S. cerevisiae. Cyclin E showed genetic interactions with the CDC28 gene, suggesting that it functioned at START by interacting with the CDC28 protein. Two human genes were identified that could interact with cyclin E to perform START in yeast containing a cdc28 mutation. One was CDC2-HS, and the second was the human homolog of Xenopus CDK2. Cyclin E produced in E. coli bound and activated the CDC2 protein in extracts from human G1 cells, and antibodies against cyclin E immunoprecipitated a histone H1 kinase from HeLa cells. The interactions between cyclin E and CDC2, or CDK2, may be important at the G1 to S transition in human cells.
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U2 - 10.1016/0092-8674(91)90044-Y
DO - 10.1016/0092-8674(91)90044-Y
M3 - Article
C2 - 1833068
AN - SCOPUS:0025734356
SN - 0092-8674
VL - 66
SP - 1217
EP - 1228
JO - Cell
JF - Cell
IS - 6
ER -