TY - JOUR
T1 - Human Erythrocyte Membrane Protein 4.2 is Palmitoylated
AU - Das, Amit K.
AU - Bhattacharya, Raja
AU - Kundu, Manikuntala
AU - Chakrabarti, Parul
AU - Basu, Joyoti
PY - 1994/9
Y1 - 1994/9
N2 - Protein 4.2 is a major protein of the human erythrocyte membrane. It has previously been shown to be N‐myristoylated. After labeling of intact human erythrocytes with [3H]palmitic acid, radioactivity was found to be associated with protein 4.2 by immunoprecipitation of peripheral membrane proteins extracted at pH 11 from ghosts with anti‐(4.2) sera, followed by SDS/PAGE and fluorography. The fatty acid linked to protein 4.2 was identified as palmitic acid after hydrolysis of protein and thin‐layer chromatography of the fatty acid extracted in the organic phase. Protein 4.2 could be depalmitoylated with hydroxylamine, suggesting a thioester linkage. Depalmitoylated protein 4.2 showed significantly decreased binding to protein‐4.2‐depleted membranes, compared to native protein 4.2.
AB - Protein 4.2 is a major protein of the human erythrocyte membrane. It has previously been shown to be N‐myristoylated. After labeling of intact human erythrocytes with [3H]palmitic acid, radioactivity was found to be associated with protein 4.2 by immunoprecipitation of peripheral membrane proteins extracted at pH 11 from ghosts with anti‐(4.2) sera, followed by SDS/PAGE and fluorography. The fatty acid linked to protein 4.2 was identified as palmitic acid after hydrolysis of protein and thin‐layer chromatography of the fatty acid extracted in the organic phase. Protein 4.2 could be depalmitoylated with hydroxylamine, suggesting a thioester linkage. Depalmitoylated protein 4.2 showed significantly decreased binding to protein‐4.2‐depleted membranes, compared to native protein 4.2.
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U2 - 10.1111/j.1432-1033.1994.00575.x
DO - 10.1111/j.1432-1033.1994.00575.x
M3 - Article
C2 - 7925374
AN - SCOPUS:0028108225
SN - 0014-2956
VL - 224
SP - 575
EP - 580
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 2
ER -