@article{7886459548bf4440bee51d7c1c323c5f,
title = "Human LBP-32/MGR is a Repressor of the P450scc in Human Choriocarcinoma Cell Line JEG-3",
abstract = "Steroid hormones regulate a wide range of physiologic functions in humans. The cholesterol side-chain cleavage enzyme P450scc regulates the initial step of biosynthesis of all steroid hormones. We investigated the expression of P450scc by studying a potential regulator of P450scc, LBP-32/MGR. Using a Northern blot, we found that LBP-32/MGR mRNA was expressed mainly in the human placenta. Using radiation hybrid mapping, we identified LBP-32/MGR on human chromosome 2p25. Recombinant LBP-32/MGR protein bound preferentially to a DNA fragment from the promoter of P450scc in vitro and exhibited clear nuclear localization in transfected cells. Luciferase reporter gene assays showed that LBP-32/MGR specifically repressed transcriptional activation of the human P450scc promoter. Because placental P450scc expression is essential for pregnancy and steroid biosynthesis, the placental expression and transcriptional repressor activity of LBP-32/MGR in JEG-3 cells suggest it has a role as a transcriptional modulator of steroid biosynthesis.",
keywords = "JEG-3, LBP-32, Mammalian grainyhead, P450scc, Transcriptional repressor",
author = "Henderson, {Y. C.} and Frederick, {M. J.} and A. Jayakumar and Y. Choi and Wang, {M. T.} and Y. Kang and R. Evans and Spring, {P. M.} and M. Uesugi and Clayman, {G. L.}",
note = "Funding Information: This work was supported in part by National Institutes of Health Independent Award R01 DE-13954 (G. L. C), National Institutes of Health Specialized Program of Research Excellence (SPORE) grant in Head and Neck Cancer 1P50-CA-97007, M. D. Anderson Cancer Center Support grant 5P30 CA-16672, the Michael A. O'Bannon Endowment for Cancer Research, the Betty Berry Cancer Research Fund, funds from the M. D. Anderson Cancer Center's Tobacco Research Program of the Texas Legislative Tobacco Settlement, and Cancer Center Core NCI Grant CA-16672 for DNA sequencing and media production. We wish to thank the University of Iowa Gene Transfer Vector Core, supported in part by the NIH and the Roy J. Carver Foundation, for adenoviral vector preparation. We wish to thank Dr. Douglas Boyd, Dr. Rakesh Kumar, and David Galloway for critically reviewing and editing the manuscript; Dr. El-Naggar for providing the ABI Prism 7900HT machine and SDS software; Dr. Dianna Roberts for statistical analysis; Wendy Schober-Ditmore for flow cytometry; Dr. Torahiko Nakashima, Dr. Hernan Gonzalez, Dr. Kenji Mitsudo, Katrina Briggs, Paula Holton, and Kelli Cottingham for technical support; and Sonia Perez for manuscript preparation.",
year = "2007",
month = feb,
doi = "10.1016/j.placenta.2006.03.008",
language = "English (US)",
volume = "28",
pages = "152--160",
journal = "Placenta",
issn = "0143-4004",
publisher = "W.B. Saunders Ltd",
number = "2-3",
}