Human papillomavirus and Epstein-Barr virus infections in breast cancer from chile

Francisco Aguayo; Noureen Khan; Chihaya Koriyama; Carolina González; Sandra Ampuero; Oslando Padilla; Luisa Solís; Yoshito Eizuru; Alejandro Corvalán; Suminori Akiba

doi:10.1186/1750-9378-6-7

Human papillomavirus and Epstein-Barr virus infections in breast cancer from chile

Francisco Aguayo, Noureen Khan, Chihaya Koriyama, Carolina González, Sandra Ampuero, Oslando Padilla, Luisa Solís, Yoshito Eizuru, Alejandro Corvalán, Suminori Akiba

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

Background: Human papillomavirus (HPV) and Epstein Barr virus (EBV) have been found in breast carcinomas (BCs) around the world. In this study, fifty-five BCs from Chile were analyzed for HPV and EBV presence. In addition, HPV-16 viral load/physical status and E6/E7 expressions were determined. Results: The amplification of a housekeeping gene showed that 46/55 samples (84%) had amplifiable DNA. HPV-16 was detected in 4/46 BCs (8.7%) and EBV was detected in 3/46 (6.5%) BCs. The analysis of HPV-16 physical status showed that this virus was integrated in all of the tumors with a relatively low viral load (range: 0.14 to 33.8 copies/cell). E6 and E7 transcripts, however, were not detected in any HPV-16 positive specimens. Using a Cox-regression model, we found a statistically significant association between EBV presence and poor survival (p = 0.013). Conclusions: The findings in this study suggest that it is unlikely that HPV and/or EBV play a direct role in the etiology of BC.

Original language	English (US)
Article number	7
Journal	Infectious Agents and Cancer
Volume	6
Issue number	1
DOIs	https://doi.org/10.1186/1750-9378-6-7
State	Published - 2011
Externally published	Yes

Keywords

HPV
breast
cancer
integration
papillomavirus

ASJC Scopus subject areas

Epidemiology
Oncology
Cancer Research
Infectious Diseases

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10.1186/1750-9378-6-7

Cite this

@article{86194c41766d44faa58d3f9148fcaba0,

title = "Human papillomavirus and Epstein-Barr virus infections in breast cancer from chile",

abstract = "Background: Human papillomavirus (HPV) and Epstein Barr virus (EBV) have been found in breast carcinomas (BCs) around the world. In this study, fifty-five BCs from Chile were analyzed for HPV and EBV presence. In addition, HPV-16 viral load/physical status and E6/E7 expressions were determined. Results: The amplification of a housekeeping gene showed that 46/55 samples (84%) had amplifiable DNA. HPV-16 was detected in 4/46 BCs (8.7%) and EBV was detected in 3/46 (6.5%) BCs. The analysis of HPV-16 physical status showed that this virus was integrated in all of the tumors with a relatively low viral load (range: 0.14 to 33.8 copies/cell). E6 and E7 transcripts, however, were not detected in any HPV-16 positive specimens. Using a Cox-regression model, we found a statistically significant association between EBV presence and poor survival (p = 0.013). Conclusions: The findings in this study suggest that it is unlikely that HPV and/or EBV play a direct role in the etiology of BC.",

keywords = "HPV, breast, cancer, integration, papillomavirus",

author = "Francisco Aguayo and Noureen Khan and Chihaya Koriyama and Carolina Gonz{\'a}lez and Sandra Ampuero and Oslando Padilla and Luisa Sol{\'i}s and Yoshito Eizuru and Alejandro Corval{\'a}n and Suminori Akiba",

note = "Funding Information: This work was financed by Grants-in-Aid for Scientific Research on Priority areas (12218231 and 17015037) of the Ministry of Education, Culture, Sports, Science and Technology, Japan. Work in Chile was supported by Fondecyt Grant 11080198 to Francisco Aguayo and 1080563 to Alejandro Corval{\'a}n. We thank to Maria Lina Tornesello, Ph.D. from Instituto Nazionale Tumori “Fond. G. Pascale”, Naples, Italy, for proofreading the manuscript.",

year = "2011",

doi = "10.1186/1750-9378-6-7",

language = "English (US)",

volume = "6",

journal = "Infectious Agents and Cancer",

issn = "1750-9378",

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TY - JOUR

T1 - Human papillomavirus and Epstein-Barr virus infections in breast cancer from chile

AU - Aguayo, Francisco

AU - Khan, Noureen

AU - Koriyama, Chihaya

AU - González, Carolina

AU - Ampuero, Sandra

AU - Padilla, Oslando

AU - Solís, Luisa

AU - Eizuru, Yoshito

AU - Corvalán, Alejandro

AU - Akiba, Suminori

N1 - Funding Information: This work was financed by Grants-in-Aid for Scientific Research on Priority areas (12218231 and 17015037) of the Ministry of Education, Culture, Sports, Science and Technology, Japan. Work in Chile was supported by Fondecyt Grant 11080198 to Francisco Aguayo and 1080563 to Alejandro Corvalán. We thank to Maria Lina Tornesello, Ph.D. from Instituto Nazionale Tumori “Fond. G. Pascale”, Naples, Italy, for proofreading the manuscript.

PY - 2011

Y1 - 2011

N2 - Background: Human papillomavirus (HPV) and Epstein Barr virus (EBV) have been found in breast carcinomas (BCs) around the world. In this study, fifty-five BCs from Chile were analyzed for HPV and EBV presence. In addition, HPV-16 viral load/physical status and E6/E7 expressions were determined. Results: The amplification of a housekeeping gene showed that 46/55 samples (84%) had amplifiable DNA. HPV-16 was detected in 4/46 BCs (8.7%) and EBV was detected in 3/46 (6.5%) BCs. The analysis of HPV-16 physical status showed that this virus was integrated in all of the tumors with a relatively low viral load (range: 0.14 to 33.8 copies/cell). E6 and E7 transcripts, however, were not detected in any HPV-16 positive specimens. Using a Cox-regression model, we found a statistically significant association between EBV presence and poor survival (p = 0.013). Conclusions: The findings in this study suggest that it is unlikely that HPV and/or EBV play a direct role in the etiology of BC.

AB - Background: Human papillomavirus (HPV) and Epstein Barr virus (EBV) have been found in breast carcinomas (BCs) around the world. In this study, fifty-five BCs from Chile were analyzed for HPV and EBV presence. In addition, HPV-16 viral load/physical status and E6/E7 expressions were determined. Results: The amplification of a housekeeping gene showed that 46/55 samples (84%) had amplifiable DNA. HPV-16 was detected in 4/46 BCs (8.7%) and EBV was detected in 3/46 (6.5%) BCs. The analysis of HPV-16 physical status showed that this virus was integrated in all of the tumors with a relatively low viral load (range: 0.14 to 33.8 copies/cell). E6 and E7 transcripts, however, were not detected in any HPV-16 positive specimens. Using a Cox-regression model, we found a statistically significant association between EBV presence and poor survival (p = 0.013). Conclusions: The findings in this study suggest that it is unlikely that HPV and/or EBV play a direct role in the etiology of BC.

KW - HPV

KW - breast

KW - cancer

KW - integration

KW - papillomavirus

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ER -

Human papillomavirus and Epstein-Barr virus infections in breast cancer from chile

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