Human Papillomavirus DNA in LEEP Plume

Anil K. Sood; I. Keith Stone; Zahra Bahrani-Mostafavi; Jay Stoerker

doi:10.1155/S1064744994000591

Human Papillomavirus DNA in LEEP Plume

Anil K. Sood, I. Keith Stone, Zahra Bahrani-Mostafavi, Jay Stoerker

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Objective: This study was undertaken to determine the prevalence of human papillomavirus (HPV) in loop electrosurgical excision procedure (LEEP) plumes. Methods: Forty-nine consecutive patients with colposcopic and cytologic evidence of cervical intraepithelial neoplasia (CIN) were tested. Smoke plumes were collected through a filter placed in the suction tubing. DNA was harvested by proteinase K digest of the filters and prepared for polymerase chain reaction (PCR) by L1 consensus primers. Results: Thirty-nine (80%) tissue samples were positive for HPV, with types 6/11 in 4, 16/18 in 19, 31/33/35 in 2, and other types in 6 patients. The tissue sample was inadequate for typing in 8 patients. HPV DNA was detected in 18 (37%) filters. Conclusions: Although the consequences of HPV in LEEP plume are unknown, it would be prudent to adopt stringent control procedures.

Original language	English (US)
Pages (from-to)	167-170
Number of pages	4
Journal	Infectious Diseases in Obstetrics and Gynecology
Volume	2
Issue number	4
DOIs	https://doi.org/10.1155/S1064744994000591
State	Published - 1994
Externally published	Yes

Keywords

Cervical intraepithelial neoplasia
HPV DNA
loop electrosurgical excision

ASJC Scopus subject areas

Dermatology
Obstetrics and Gynecology
Infectious Diseases

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10.1155/S1064744994000591

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title = "Human Papillomavirus DNA in LEEP Plume",

abstract = "Objective: This study was undertaken to determine the prevalence of human papillomavirus (HPV) in loop electrosurgical excision procedure (LEEP) plumes. Methods: Forty-nine consecutive patients with colposcopic and cytologic evidence of cervical intraepithelial neoplasia (CIN) were tested. Smoke plumes were collected through a filter placed in the suction tubing. DNA was harvested by proteinase K digest of the filters and prepared for polymerase chain reaction (PCR) by L1 consensus primers. Results: Thirty-nine (80%) tissue samples were positive for HPV, with types 6/11 in 4, 16/18 in 19, 31/33/35 in 2, and other types in 6 patients. The tissue sample was inadequate for typing in 8 patients. HPV DNA was detected in 18 (37%) filters. Conclusions: Although the consequences of HPV in LEEP plume are unknown, it would be prudent to adopt stringent control procedures.",

keywords = "Cervical intraepithelial neoplasia, HPV DNA, loop electrosurgical excision",

author = "Sood, {Anil K.} and {Keith Stone}, I. and Zahra Bahrani-Mostafavi and Jay Stoerker",

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doi = "10.1155/S1064744994000591",

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number = "4",

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T1 - Human Papillomavirus DNA in LEEP Plume

AU - Sood, Anil K.

AU - Keith Stone, I.

AU - Bahrani-Mostafavi, Zahra

AU - Stoerker, Jay

PY - 1994

Y1 - 1994

N2 - Objective: This study was undertaken to determine the prevalence of human papillomavirus (HPV) in loop electrosurgical excision procedure (LEEP) plumes. Methods: Forty-nine consecutive patients with colposcopic and cytologic evidence of cervical intraepithelial neoplasia (CIN) were tested. Smoke plumes were collected through a filter placed in the suction tubing. DNA was harvested by proteinase K digest of the filters and prepared for polymerase chain reaction (PCR) by L1 consensus primers. Results: Thirty-nine (80%) tissue samples were positive for HPV, with types 6/11 in 4, 16/18 in 19, 31/33/35 in 2, and other types in 6 patients. The tissue sample was inadequate for typing in 8 patients. HPV DNA was detected in 18 (37%) filters. Conclusions: Although the consequences of HPV in LEEP plume are unknown, it would be prudent to adopt stringent control procedures.

AB - Objective: This study was undertaken to determine the prevalence of human papillomavirus (HPV) in loop electrosurgical excision procedure (LEEP) plumes. Methods: Forty-nine consecutive patients with colposcopic and cytologic evidence of cervical intraepithelial neoplasia (CIN) were tested. Smoke plumes were collected through a filter placed in the suction tubing. DNA was harvested by proteinase K digest of the filters and prepared for polymerase chain reaction (PCR) by L1 consensus primers. Results: Thirty-nine (80%) tissue samples were positive for HPV, with types 6/11 in 4, 16/18 in 19, 31/33/35 in 2, and other types in 6 patients. The tissue sample was inadequate for typing in 8 patients. HPV DNA was detected in 18 (37%) filters. Conclusions: Although the consequences of HPV in LEEP plume are unknown, it would be prudent to adopt stringent control procedures.

KW - Cervical intraepithelial neoplasia

KW - HPV DNA

KW - loop electrosurgical excision

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Human Papillomavirus DNA in LEEP Plume

Abstract

Keywords

ASJC Scopus subject areas

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