TY - JOUR
T1 - Human sickle hemoglobin in transgenic mice
AU - Ryan, Thomas M.
AU - Townes, Tim M.
AU - Reilly, Michael P.
AU - Asakura, Toshio
AU - Palmiter, Richard D.
AU - Brinster, Ralph L.
AU - Behringer, Richard R.
PY - 1990
Y1 - 1990
N2 - DNA molecules that contain the human α- and βs-globin genes inserted downstream of erythroid-specific, deoxyribonudease I super-hypersensitive sites were coinjected into fertilized mouse eggs and a transgenic mouse line was established that synthesizes human sickle hemoglobin (Hb S). These animals were bred to β-thalassemic mice to reduce endogenous mouse globin levels. When erythrocytes from these mice were deoxygenated, greater than 90 percent of the cells displayed the same characteristic sickled shapes as erythrocytes from humans with sickle cell disease. Compared to controls the mice have decreased hematocrits, elevated reticulocyte counts, lower hemoglobin concentrations, and splenomegaly, which are all indications of the anemia associated with human sickle cell disease.
AB - DNA molecules that contain the human α- and βs-globin genes inserted downstream of erythroid-specific, deoxyribonudease I super-hypersensitive sites were coinjected into fertilized mouse eggs and a transgenic mouse line was established that synthesizes human sickle hemoglobin (Hb S). These animals were bred to β-thalassemic mice to reduce endogenous mouse globin levels. When erythrocytes from these mice were deoxygenated, greater than 90 percent of the cells displayed the same characteristic sickled shapes as erythrocytes from humans with sickle cell disease. Compared to controls the mice have decreased hematocrits, elevated reticulocyte counts, lower hemoglobin concentrations, and splenomegaly, which are all indications of the anemia associated with human sickle cell disease.
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U2 - 10.1126/science.2154033
DO - 10.1126/science.2154033
M3 - Article
C2 - 2154033
AN - SCOPUS:0025191104
SN - 0036-8075
VL - 247
SP - 566
EP - 568
JO - Science
JF - Science
IS - 4942
ER -