TY - JOUR
T1 - Identification and cDNA cloning of headpin, a novel differentially expressed serpin that maps to chromosome 18q
AU - Spring, Paul
AU - Nakashima, Torahiko
AU - Frederick, Mitchell
AU - Henderson, Ying
AU - Clayman, Gary
N1 - Funding Information:
This work was supported in part by Grant R29 DE11689-01A1 from the National Institute of Health First Investigator Award (G.L.C.), Grant 1P50DE11906-01, Oral Cancer Research Center of Excellence Award of the National Institute of Dental and Craniofacial Research (G.L.C.), and Grant NIH-NCI-CA 16672 from the Cancer Center Support Grant. The GenBank accession number given to headpin is AF169949. 1To whom correspondence should be addressed at Department of Head and Neck Surgery, University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Blvd., Box 069, Houston, TX 77030. E-mail: gclayman@notes.mdacc.tmc.edu.
PY - 1999/10/14
Y1 - 1999/10/14
N2 - Differential display was used to identify a novel serpin (headpin) underexpressed in squamous cell cancers of the oral cavity. Headpin cDNA encoding a complete open reading frame was cloned and sequenced. Headpin is expressed in normal oral mucosal tissue, skin, and cultured keratinocytes. Using Northern analysis and relative reverse-transcription polymerase chain reaction (relative RT-PCR), downregulation of headpin mRNA expression was demonstrated in oral cavity squamous carcinomas. Northern blot analysis identified a 3.3-kb headpin mRNA transcript. Headpin is a 391-amino-acid protein with a theoretical molecular weight of 44 kDa. Hinge region homology at the reactive site loop suggests that headpin belongs to the inhibitory class of serine protease inhibitors. Headpin was mapped to 18q21.3/18q22. This region includes the ovalbumin serpins (ov-serpins) maspin, SCCA1, SCCA2, and PAI-2. Furthermore, 18q is recognized as a region for frequent loss of heterozygosity (LOH) in head and neck cancer and other malignancies.
AB - Differential display was used to identify a novel serpin (headpin) underexpressed in squamous cell cancers of the oral cavity. Headpin cDNA encoding a complete open reading frame was cloned and sequenced. Headpin is expressed in normal oral mucosal tissue, skin, and cultured keratinocytes. Using Northern analysis and relative reverse-transcription polymerase chain reaction (relative RT-PCR), downregulation of headpin mRNA expression was demonstrated in oral cavity squamous carcinomas. Northern blot analysis identified a 3.3-kb headpin mRNA transcript. Headpin is a 391-amino-acid protein with a theoretical molecular weight of 44 kDa. Hinge region homology at the reactive site loop suggests that headpin belongs to the inhibitory class of serine protease inhibitors. Headpin was mapped to 18q21.3/18q22. This region includes the ovalbumin serpins (ov-serpins) maspin, SCCA1, SCCA2, and PAI-2. Furthermore, 18q is recognized as a region for frequent loss of heterozygosity (LOH) in head and neck cancer and other malignancies.
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U2 - 10.1006/bbrc.1999.1453
DO - 10.1006/bbrc.1999.1453
M3 - Article
C2 - 10527881
AN - SCOPUS:0033554571
SN - 0006-291X
VL - 264
SP - 299
EP - 304
JO - Biochemical and biophysical research communications
JF - Biochemical and biophysical research communications
IS - 1
ER -