TY - JOUR
T1 - Identification and clinical impact of potentially actionable somatic oncogenic mutations in solid tumor samples
AU - Toomey, Sinead
AU - Carr, Aoife
AU - Mezynski, Mateusz Janusz
AU - Elamin, Yasir
AU - Rafee, Shereen
AU - Cremona, Mattia
AU - Morgan, Clare
AU - Madden, Stephen
AU - Abdul-Jalil, Khairun I.
AU - Gately, Kathy
AU - Farrelly, Angela
AU - Kay, Elaine W.
AU - Kennedy, Susan
AU - O'Byrne, Kenneth
AU - Grogan, Liam
AU - Breathnach, Oscar
AU - Morris, Patrick G.
AU - Eustace, Alexander J.
AU - Fay, Joanna
AU - Cummins, Robert
AU - O'Grady, Anthony
AU - Kalachand, Roshni
AU - O'Donovan, Norma
AU - Kelleher, Fergal
AU - O'Reilly, Aine
AU - Doherty, Mark
AU - Crown, John
AU - Hennessy, Bryan T.
N1 - Publisher Copyright:
© 2020 The Author(s).
PY - 2020/2/22
Y1 - 2020/2/22
N2 - Background: An increasing number of anti-cancer therapeutic agents target specific mutant proteins that are expressed by many different tumor types. Successful use of these therapies is dependent on the presence or absence of somatic mutations within the patient's tumor that can confer clinical efficacy or drug resistance. Methods: The aim of our study was to determine the type, frequency, overlap and functional proteomic effects of potentially targetable recurrent somatic hotspot mutations in 47 cancer-related genes in multiple disease sites that could be potential therapeutic targets using currently available agents or agents in clinical development. Results: Using MassArray technology, of the 1300 patient tumors analysed 571 (43.9%) had at least one somatic mutation. Mutations were identified in 30 different genes. KRAS (16.5%), PIK3CA (13.6%) and BRAF (3.8%) were the most frequently mutated genes. Prostate (10.8%) had the lowest number of somatic mutations identified, while no mutations were identified in sarcoma. Ocular melanoma (90.6%), endometrial (72.4%) and colorectal (66.4%) tumors had the highest number of mutations. We noted high concordance between mutations in different parts of the tumor (94%) and matched primary and metastatic samples (90%). KRAS and BRAF mutations were mutually exclusive. Mutation co-occurrence involved mainly PIK3CA and PTPN11, and PTPN11 and APC. Reverse Phase Protein Array (RPPA) analysis demonstrated that PI3K and MAPK signalling pathways were more altered in tumors with mutations compared to wild type tumors. Conclusions: Hotspot mutational profiling is a sensitive, high-Throughput approach for identifying mutations of clinical relevance to molecular based therapeutics for treatment of cancer, and could potentially be of use in identifying novel opportunities for genotype-driven clinical trials.
AB - Background: An increasing number of anti-cancer therapeutic agents target specific mutant proteins that are expressed by many different tumor types. Successful use of these therapies is dependent on the presence or absence of somatic mutations within the patient's tumor that can confer clinical efficacy or drug resistance. Methods: The aim of our study was to determine the type, frequency, overlap and functional proteomic effects of potentially targetable recurrent somatic hotspot mutations in 47 cancer-related genes in multiple disease sites that could be potential therapeutic targets using currently available agents or agents in clinical development. Results: Using MassArray technology, of the 1300 patient tumors analysed 571 (43.9%) had at least one somatic mutation. Mutations were identified in 30 different genes. KRAS (16.5%), PIK3CA (13.6%) and BRAF (3.8%) were the most frequently mutated genes. Prostate (10.8%) had the lowest number of somatic mutations identified, while no mutations were identified in sarcoma. Ocular melanoma (90.6%), endometrial (72.4%) and colorectal (66.4%) tumors had the highest number of mutations. We noted high concordance between mutations in different parts of the tumor (94%) and matched primary and metastatic samples (90%). KRAS and BRAF mutations were mutually exclusive. Mutation co-occurrence involved mainly PIK3CA and PTPN11, and PTPN11 and APC. Reverse Phase Protein Array (RPPA) analysis demonstrated that PI3K and MAPK signalling pathways were more altered in tumors with mutations compared to wild type tumors. Conclusions: Hotspot mutational profiling is a sensitive, high-Throughput approach for identifying mutations of clinical relevance to molecular based therapeutics for treatment of cancer, and could potentially be of use in identifying novel opportunities for genotype-driven clinical trials.
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U2 - 10.1186/s12967-020-02273-4
DO - 10.1186/s12967-020-02273-4
M3 - Article
C2 - 32087721
AN - SCOPUS:85079748284
SN - 1479-5876
VL - 18
JO - Journal of translational medicine
JF - Journal of translational medicine
IS - 1
M1 - 99
ER -