Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC)

Hongjun Shu; Yingxin Zhao; Ge Zhou; She Chen; Julia D. Wulfkuhle; Dennis C. Sgroi; Henry Kruztsch; Kelly McLean; Melodie Knowlton; Aysegul Sahin; Yue Chen; Emanuel Petricoin; Patricia S. Steeg; Yingming Zhao

Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC)

Hongjun Shu, Yingxin Zhao, Ge Zhou, She Chen, Julia D. Wulfkuhle, Dennis C. Sgroi, Henry Kruztsch, Kelly McLean, Melodie Knowlton, Aysegul Sahin, Yue Chen, Emanuel Petricoin, Patricia S. Steeg, Yingming Zhao

Research output: Contribution to conference › Paper › peer-review

Abstract

A proteomic comparison of matched normal and cancer cells was presented, and the new facts of the transition of breast and esophageal cancer were revealed. Pure populations of normal and cancer cells were obtained using laser capture microdissection (LCM). Protein sample preparation, 2D-gel electrophoresis, and mass spectrometry of protein identification, and immunohistochemistry were performed. It was found that the 3D simulation of up and down-regulated protein spots. Results show the spot pairs of up-regulated fascin, equally expressed tubulin and down regulated annexin.

Original language	English (US)
Pages	227-228
Number of pages	2
State	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Cite this

Shu, H., Zhao, Y., Zhou, G., Chen, S., Wulfkuhle, J. D., Sgroi, D. C., Kruztsch, H., McLean, K., Knowlton, M., Sahin, A., Chen, Y., Petricoin, E., Steeg, P. S., & Zhao, Y. (2002). Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC). 227-228. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC). / Shu, Hongjun; Zhao, Yingxin; Zhou, Ge et al.
2002. 227-228 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conference › Paper › peer-review

Shu, H, Zhao, Y, Zhou, G, Chen, S, Wulfkuhle, JD, Sgroi, DC, Kruztsch, H, McLean, K, Knowlton, M, Sahin, A, Chen, Y, Petricoin, E, Steeg, PS & Zhao, Y 2002, 'Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC)', Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02 - 6/6/02 pp. 227-228.

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title = "Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC)",

abstract = "A proteomic comparison of matched normal and cancer cells was presented, and the new facts of the transition of breast and esophageal cancer were revealed. Pure populations of normal and cancer cells were obtained using laser capture microdissection (LCM). Protein sample preparation, 2D-gel electrophoresis, and mass spectrometry of protein identification, and immunohistochemistry were performed. It was found that the 3D simulation of up and down-regulated protein spots. Results show the spot pairs of up-regulated fascin, equally expressed tubulin and down regulated annexin.",

author = "Hongjun Shu and Yingxin Zhao and Ge Zhou and She Chen and Wulfkuhle, {Julia D.} and Sgroi, {Dennis C.} and Henry Kruztsch and Kelly McLean and Melodie Knowlton and Aysegul Sahin and Yue Chen and Emanuel Petricoin and Steeg, {Patricia S.} and Yingming Zhao",

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T1 - Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC)

AU - Shu, Hongjun

AU - Zhao, Yingxin

AU - Zhou, Ge

AU - Chen, She

AU - Wulfkuhle, Julia D.

AU - Sgroi, Dennis C.

AU - Kruztsch, Henry

AU - McLean, Kelly

AU - Knowlton, Melodie

AU - Sahin, Aysegul

AU - Chen, Yue

AU - Petricoin, Emanuel

AU - Steeg, Patricia S.

AU - Zhao, Yingming

PY - 2002

Y1 - 2002

N2 - A proteomic comparison of matched normal and cancer cells was presented, and the new facts of the transition of breast and esophageal cancer were revealed. Pure populations of normal and cancer cells were obtained using laser capture microdissection (LCM). Protein sample preparation, 2D-gel electrophoresis, and mass spectrometry of protein identification, and immunohistochemistry were performed. It was found that the 3D simulation of up and down-regulated protein spots. Results show the spot pairs of up-regulated fascin, equally expressed tubulin and down regulated annexin.

AB - A proteomic comparison of matched normal and cancer cells was presented, and the new facts of the transition of breast and esophageal cancer were revealed. Pure populations of normal and cancer cells were obtained using laser capture microdissection (LCM). Protein sample preparation, 2D-gel electrophoresis, and mass spectrometry of protein identification, and immunohistochemistry were performed. It was found that the 3D simulation of up and down-regulated protein spots. Results show the spot pairs of up-regulated fascin, equally expressed tubulin and down regulated annexin.

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M3 - Paper

AN - SCOPUS:2442710317

SP - 227

EP - 228

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC)

Abstract

Conference

ASJC Scopus subject areas

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