TY - JOUR
T1 - Identification of EpCAM as a molecular target of prostate cancer stroma
AU - Mukherjee, Sumana
AU - Richardson, Annely M.
AU - Rodriguez-Canales, Jaime
AU - Ylaya, Kris
AU - Erickson, Heidi S.
AU - Player, Audrey
AU - Kawasaki, Ernest S.
AU - Pinto, Peter A.
AU - Choyke, Peter L.
AU - Merino, Maria J.
AU - Albert, Paul S.
AU - Chuaqui, Rodrigo F.
AU - Emmert-Buck, Michael R.
N1 - Funding Information:
Supported in part by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research.
PY - 2009
Y1 - 2009
N2 - To delineate the molecular changes that occur in the tumor microenvironment, we previously performed global transcript analysis of human prostate cancer specimens using tissue microdissection and expression microarrays. Epithelial and stromal compartments were individually studied in both tumor and normal fields. Tumor-associated stroma showed a distinctly different expression pattern compared with normal stroma, having 44 differentially expressed transcripts, the majority of which were up-regulated. In the present study, one of the up-regulated transcripts, epithelial cell adhesion activating molecule, was further evaluated at the protein level in 20 prostate cancer cases using immunohistochemistry and a histomathematical analysis strategy. The epithelial cell adhesion activating molecule showed a 76-fold expression increase in the tumor-associated stroma, as compared with matched normal stroma. Moreover, Gleason 4 or 5 tumor stroma was increased 170-fold relative to matched normal stroma, whereas the Gleason 3 tumor area showed only a 36-fold increase, indicating a positive correlation with Gleason tumor grade. Since the stromal compartment may be particularly accessible to vascular-delivered agents, epithelial cell adhesion activating molecule could become a valuable molecular target for imaging or treatment of prostate cancer.
AB - To delineate the molecular changes that occur in the tumor microenvironment, we previously performed global transcript analysis of human prostate cancer specimens using tissue microdissection and expression microarrays. Epithelial and stromal compartments were individually studied in both tumor and normal fields. Tumor-associated stroma showed a distinctly different expression pattern compared with normal stroma, having 44 differentially expressed transcripts, the majority of which were up-regulated. In the present study, one of the up-regulated transcripts, epithelial cell adhesion activating molecule, was further evaluated at the protein level in 20 prostate cancer cases using immunohistochemistry and a histomathematical analysis strategy. The epithelial cell adhesion activating molecule showed a 76-fold expression increase in the tumor-associated stroma, as compared with matched normal stroma. Moreover, Gleason 4 or 5 tumor stroma was increased 170-fold relative to matched normal stroma, whereas the Gleason 3 tumor area showed only a 36-fold increase, indicating a positive correlation with Gleason tumor grade. Since the stromal compartment may be particularly accessible to vascular-delivered agents, epithelial cell adhesion activating molecule could become a valuable molecular target for imaging or treatment of prostate cancer.
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U2 - 10.2353/ajpath.2009.090013
DO - 10.2353/ajpath.2009.090013
M3 - Article
C2 - 19850885
AN - SCOPUS:73549124547
SN - 0002-9440
VL - 175
SP - 2277
EP - 2287
JO - American Journal of Pathology
JF - American Journal of Pathology
IS - 6
ER -