Abstract
Accurate understanding of cellular processes and responses to stimuli is of paramount importance in biomedical research and diagnosis. Raman spectroscopy (RS), a label-free and nondestructive spectroscopic method has the potential to serve as a novel ’theranostics' tool. Both fiber-optic and micro-Raman studies have demonstrated efficacy in diagnostics and therapeutic response monitoring. In the present study, we have evaluated the potential of micro-Raman spectroscopic maps in identifying changes induced by loss of K8/18 proteins in a tongue cancer cell line. Furthermore, we also evaluated the efficacy of less expensive and commercially available fiber probes to identify K8/18 wild and knock-down cell pellets, in view of the utility of cell pellet-based studies. The findings suggest that major differences in the cellular morphology and biochemical composition can be objectively identified and can be utilized for classification using both micro-Raman and fiber-probe-based RS. These findings highlight the potential of fiber-optic probe-based RS in noninvasive cellular phenotyping for diagnosis and therapeutic response monitoring, especially in low-resource settings. (Figure presented.).
Original language | English (US) |
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Pages (from-to) | 1377-1384 |
Number of pages | 8 |
Journal | Journal of Biophotonics |
Volume | 10 |
Issue number | 10 |
DOIs | |
State | Published - Oct 2017 |
Keywords
- Raman spectroscopy
- biophotonics
- keratin
- oral cancers
- wild and single knockdown cells
ASJC Scopus subject areas
- General Chemistry
- General Materials Science
- General Biochemistry, Genetics and Molecular Biology
- General Engineering
- General Physics and Astronomy