Identification of novel chicken microRNAs and analysis of their genomic organization

Peng Shao; Hui Zhou; Zhen Dong Xiao; Jie Hua He; Mian Bo Huang; Yue Qin Chen; Liang Hu Qu

doi:10.1016/j.gene.2008.04.004

Identification of novel chicken microRNAs and analysis of their genomic organization

Peng Shao, Hui Zhou, Zhen Dong Xiao, Jie Hua He, Mian Bo Huang, Yue Qin Chen, Liang Hu Qu

Experimental Radiation Oncology

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

MicroRNAs (miRNAs) represent a family of small noncoding RNAs with important regulatory roles in diverse biological processes ranging from cell differentiation to organism development. In chickens, the full set of miRNAs and the expression patterns of miRNAs during development are still poorly understood when compared to the other vertebrates. In this study, we identified 29 novel miRNAs and 140 potential miRNA loci in the chicken genome by combining the experimental and computational analyses. Detailed expression patterns of 49 miRNAs were first characterized by Northern blotting and indicated the cooperativity of the miRNA expression with their function in embryogenesis and organogenesis. Twenty-seven miRNA clusters were systematically evaluated in the chicken genome and diverse expression patterns for closely linked miRNAs were observed. Our results significantly expand the set of known miRNAs in the chicken and provide the basis for understanding the structural and functional evolution of miRNA genes in vertebrates.

Original language	English (US)
Pages (from-to)	34-40
Number of pages	7
Journal	Gene
Volume	418
Issue number	1-2
DOIs	https://doi.org/10.1016/j.gene.2008.04.004
State	Published - Jul 15 2008

Keywords

Chicken
Expression pattern
Gene cluster
MicroRNA

ASJC Scopus subject areas

Genetics

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10.1016/j.gene.2008.04.004

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title = "Identification of novel chicken microRNAs and analysis of their genomic organization",

abstract = "MicroRNAs (miRNAs) represent a family of small noncoding RNAs with important regulatory roles in diverse biological processes ranging from cell differentiation to organism development. In chickens, the full set of miRNAs and the expression patterns of miRNAs during development are still poorly understood when compared to the other vertebrates. In this study, we identified 29 novel miRNAs and 140 potential miRNA loci in the chicken genome by combining the experimental and computational analyses. Detailed expression patterns of 49 miRNAs were first characterized by Northern blotting and indicated the cooperativity of the miRNA expression with their function in embryogenesis and organogenesis. Twenty-seven miRNA clusters were systematically evaluated in the chicken genome and diverse expression patterns for closely linked miRNAs were observed. Our results significantly expand the set of known miRNAs in the chicken and provide the basis for understanding the structural and functional evolution of miRNA genes in vertebrates.",

keywords = "Chicken, Expression pattern, Gene cluster, MicroRNA",

author = "Peng Shao and Hui Zhou and Xiao, {Zhen Dong} and He, {Jie Hua} and Huang, {Mian Bo} and Chen, {Yue Qin} and Qu, {Liang Hu}",

note = "Funding Information: We are grateful to Jian-You Liao, Xian-Feng Zeng for RNA extraction of the chicken embryos and Xiao-Hong Chen for technical assistance. This research was supported by the National Basic Research Program (No. 2005CB724600) and the funds from the Ministry of Education of China and Guangdong Province (No. IRT0447, NSF05200303). ",

year = "2008",

month = jul,

day = "15",

doi = "10.1016/j.gene.2008.04.004",

language = "English (US)",

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journal = "Gene",

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T1 - Identification of novel chicken microRNAs and analysis of their genomic organization

AU - Shao, Peng

AU - Zhou, Hui

AU - Xiao, Zhen Dong

AU - He, Jie Hua

AU - Huang, Mian Bo

AU - Chen, Yue Qin

AU - Qu, Liang Hu

N1 - Funding Information: We are grateful to Jian-You Liao, Xian-Feng Zeng for RNA extraction of the chicken embryos and Xiao-Hong Chen for technical assistance. This research was supported by the National Basic Research Program (No. 2005CB724600) and the funds from the Ministry of Education of China and Guangdong Province (No. IRT0447, NSF05200303).

PY - 2008/7/15

Y1 - 2008/7/15

N2 - MicroRNAs (miRNAs) represent a family of small noncoding RNAs with important regulatory roles in diverse biological processes ranging from cell differentiation to organism development. In chickens, the full set of miRNAs and the expression patterns of miRNAs during development are still poorly understood when compared to the other vertebrates. In this study, we identified 29 novel miRNAs and 140 potential miRNA loci in the chicken genome by combining the experimental and computational analyses. Detailed expression patterns of 49 miRNAs were first characterized by Northern blotting and indicated the cooperativity of the miRNA expression with their function in embryogenesis and organogenesis. Twenty-seven miRNA clusters were systematically evaluated in the chicken genome and diverse expression patterns for closely linked miRNAs were observed. Our results significantly expand the set of known miRNAs in the chicken and provide the basis for understanding the structural and functional evolution of miRNA genes in vertebrates.

AB - MicroRNAs (miRNAs) represent a family of small noncoding RNAs with important regulatory roles in diverse biological processes ranging from cell differentiation to organism development. In chickens, the full set of miRNAs and the expression patterns of miRNAs during development are still poorly understood when compared to the other vertebrates. In this study, we identified 29 novel miRNAs and 140 potential miRNA loci in the chicken genome by combining the experimental and computational analyses. Detailed expression patterns of 49 miRNAs were first characterized by Northern blotting and indicated the cooperativity of the miRNA expression with their function in embryogenesis and organogenesis. Twenty-seven miRNA clusters were systematically evaluated in the chicken genome and diverse expression patterns for closely linked miRNAs were observed. Our results significantly expand the set of known miRNAs in the chicken and provide the basis for understanding the structural and functional evolution of miRNA genes in vertebrates.

KW - Chicken

KW - Expression pattern

KW - Gene cluster

KW - MicroRNA

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Identification of novel chicken microRNAs and analysis of their genomic organization

Abstract

Keywords

ASJC Scopus subject areas

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