Abstract
Far western blotting is a method of identifying protein-protein interactions. One protein of interest is immobilized on a solid support membrane, then probed with a non-antibody protein. Far western blots can be used to identify specific interacting proteins in a complex mixture of proteins. They are particularly useful for examining interactions between proteins that are difficult to analyze by other methods due to solubility problems or because they are difficult to express in cells. This method is performed totally in vitro, and the proteins of interest can be prepared in a variety of ways.
Original language | English (US) |
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Pages (from-to) | Unit 20.6 |
Journal | Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.] |
Volume | Chapter 20 |
DOIs | |
State | Published - Aug 2001 |
ASJC Scopus subject areas
- Molecular Biology