TY - JOUR
T1 - Identification of ZFR, an ancient and highly conserved murine chromosome-associated zinc finger protein
AU - Meagher, Madeleine J.
AU - Schumacher, Jill M.
AU - Lee, Keesook
AU - Holdcraft, Robert W.
AU - Edelhoff, Susanne
AU - Disteche, Christine
AU - Braun, Robert E.
N1 - Funding Information:
We thank Dr Thomas Haaf for kindly providing the human anti-synaptonemal complex sera, Glen McDonald for assistance with confocal microscopy, and Todd Berard and the Department of Pharmacology and Molecular Pharmacology Facility for oligonucleotide synthesis and DNA sequencing. This work was supported by grants from the National Institutes of Health to REB (HD27215 and HD95003) and CD (GM46883).
PY - 1999/3/4
Y1 - 1999/3/4
N2 - In a screen for RNA binding proteins expressed during murine spermatogenesis, we cloned a novel, ancient zinc finger protein possessing a region common to a small class of RNA binding proteins. Zfr (zinc finger RNA binding) encodes a protein of 1052 amino acids with three widely spaced Cys2His2 zinc fingers. Outside of the zinc fingers, ZFR shares a region that is highly conserved between several RNA binding proteins containing copies of the double-stranded RNA binding motif. By northern blotting, Zfr is expressed at highest levels within the testis, ovary and brain. Immunohistochemistry and confocal microscopy were used to show that ZFR is highly expressed during meiosis I in males and females and is chromosome associated. Zfr is also expressed in Sertoli cells in the testis and granulosa cells in the ovary where it is localized to the nucleus. Using fluorescent in situ hybridization we mapped Zfr to chromosome 15 region A. ZFR appears to be an ancient protein, as apparent homologs exist in invertebrates (D. melanogaster) nematodes (C. elegans) and humans (H. sapiens).
AB - In a screen for RNA binding proteins expressed during murine spermatogenesis, we cloned a novel, ancient zinc finger protein possessing a region common to a small class of RNA binding proteins. Zfr (zinc finger RNA binding) encodes a protein of 1052 amino acids with three widely spaced Cys2His2 zinc fingers. Outside of the zinc fingers, ZFR shares a region that is highly conserved between several RNA binding proteins containing copies of the double-stranded RNA binding motif. By northern blotting, Zfr is expressed at highest levels within the testis, ovary and brain. Immunohistochemistry and confocal microscopy were used to show that ZFR is highly expressed during meiosis I in males and females and is chromosome associated. Zfr is also expressed in Sertoli cells in the testis and granulosa cells in the ovary where it is localized to the nucleus. Using fluorescent in situ hybridization we mapped Zfr to chromosome 15 region A. ZFR appears to be an ancient protein, as apparent homologs exist in invertebrates (D. melanogaster) nematodes (C. elegans) and humans (H. sapiens).
KW - Meiosis
KW - Ovary
KW - Post-transcriptional
KW - Testis
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U2 - 10.1016/S0378-1119(98)00615-5
DO - 10.1016/S0378-1119(98)00615-5
M3 - Article
C2 - 10072773
AN - SCOPUS:0033522131
SN - 0378-1119
VL - 228
SP - 197
EP - 211
JO - Gene
JF - Gene
IS - 1-2
ER -