TY - JOUR
T1 - IFT20 is critical for collagen biosynthesis in craniofacial bone formation
AU - Yamaguchi, Hiroyuki
AU - Terajima, Masahiko
AU - Kitami, Megumi
AU - Wang, Jianbo
AU - He, Li
AU - Saeki, Makio
AU - Yamauchi, Mitsuo
AU - Komatsu, Yoshihiro
N1 - Funding Information:
We gratefully acknowledge Dr. Mark Corkins and Dr. Masaru Kaku for fruitful discussion. This study was supported in part by NIH/NIAMS R21AR060978 (M.Y.), NIH/NIDCR R01DE025897 (Y.K.) and by a fellowship from the Uehara Memorial Foundation (H.Y.).
Funding Information:
We gratefully acknowledge Dr. Mark Corkins and Dr. Masaru Kaku for fruitful discussion. This study was supported in part by NIH / NIAMS R21AR060978 (M.Y.), NIH/NIDCR R01DE025897 (Y.K.) and by a fellowship from the Uehara Memorial Foundation (H.Y.).
Publisher Copyright:
© 2020 Elsevier Inc.
PY - 2020/12/17
Y1 - 2020/12/17
N2 - Intraflagellar transport (IFT) is essential for assembling primary cilia required for bone formation. Disruption of IFT frequently leads to bone defects in humans. While it has been well studied about the function of IFT in osteogenic cell proliferation and differentiation, little is known about its role in collagen biosynthesis during bone formation. Here we show that IFT20, the smallest IFT protein in the IFT-B complex, is important for collagen biosynthesis in mice. Deletion of Ift20 in craniofacial osteoblasts displayed bone defects in the face. While collagen protein levels are unaffected by loss of Ift20, collagen cross-linking was significantly altered. In both Ift20:Wnt1-Cre and Ift20:Ocn-Cre mice the bones exhibit increased hydroxylysine-aldehyde deived cross-linking, and decreased lysine-aldehyde derived cross-linking. To obtain insight into the molecular mechanisms, we examined the expression levels of telopeptidyl lysyl hydroxylase 2 (LH2), and associated chaperone complexes. The results demonstrated that, while LH2 levels were unaffected by loss of Ift20, its chaperone, FKBP65, was significantly increased in Ift20:Wnt1-Cre and Ift20:Ocn-Cre mouse calvaria as well as femurs. These results suggest that IFT20 plays a pivotal role in collagen biosynthesis by regulating, in part, telopeptidyl lysine hydroxylation and cross-linking in bone. To the best of our knowledge, this is the first to demonstrate that the IFT components control collagen post-translational modifications. This provides a novel insight into the craniofacial bone defects associated with craniofacial skeletal ciliopathies.
AB - Intraflagellar transport (IFT) is essential for assembling primary cilia required for bone formation. Disruption of IFT frequently leads to bone defects in humans. While it has been well studied about the function of IFT in osteogenic cell proliferation and differentiation, little is known about its role in collagen biosynthesis during bone formation. Here we show that IFT20, the smallest IFT protein in the IFT-B complex, is important for collagen biosynthesis in mice. Deletion of Ift20 in craniofacial osteoblasts displayed bone defects in the face. While collagen protein levels are unaffected by loss of Ift20, collagen cross-linking was significantly altered. In both Ift20:Wnt1-Cre and Ift20:Ocn-Cre mice the bones exhibit increased hydroxylysine-aldehyde deived cross-linking, and decreased lysine-aldehyde derived cross-linking. To obtain insight into the molecular mechanisms, we examined the expression levels of telopeptidyl lysyl hydroxylase 2 (LH2), and associated chaperone complexes. The results demonstrated that, while LH2 levels were unaffected by loss of Ift20, its chaperone, FKBP65, was significantly increased in Ift20:Wnt1-Cre and Ift20:Ocn-Cre mouse calvaria as well as femurs. These results suggest that IFT20 plays a pivotal role in collagen biosynthesis by regulating, in part, telopeptidyl lysine hydroxylation and cross-linking in bone. To the best of our knowledge, this is the first to demonstrate that the IFT components control collagen post-translational modifications. This provides a novel insight into the craniofacial bone defects associated with craniofacial skeletal ciliopathies.
KW - Collagen
KW - Craniofacial bone
KW - Intraflagellar transport
KW - Mice
KW - Post-translational modifications
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U2 - 10.1016/j.bbrc.2020.09.033
DO - 10.1016/j.bbrc.2020.09.033
M3 - Article
C2 - 32988591
AN - SCOPUS:85091825708
SN - 0006-291X
VL - 533
SP - 739
EP - 744
JO - Biochemical and biophysical research communications
JF - Biochemical and biophysical research communications
IS - 4
ER -