TY - JOUR
T1 - IgE-dependent adherence and cytotoxicity of rat spleen and peritoneal cells to Litomosoides carinii microfilariae
AU - Mehta, K.
AU - Sindhu, R. K.
AU - Subrahmanyam, D.
AU - Nelson, D. S.
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1980
Y1 - 1980
N2 - Serum taken after the termination of microfilaraemia from rats infected with the filarial parasite Litomosoides carinii brought about adherence and cytotoxicity of normal rat spleen and peritoneal cells to microfilariae. The activity could be absorbed to, and eluted from, anti-rat IgE, but not anti-rat IgG, immunoabsorbent columns. Immune serum heated to 56°C for 3 hr did not cause cellular adherence or cytotoxicity; the addition of fresh normal rat serum failed to restore activity. Fresh rat serum did, however, restore activity to immune serum which was inactive after being heated to 56°C for 30 min. EDTA, EGTA and diethylcarbamazine inhibited adherence. It is concluded that IgE antibodies are responsible for cellular adherence and cytotoxicity and that complement may play a part, as yet undefined, in these reactions.
AB - Serum taken after the termination of microfilaraemia from rats infected with the filarial parasite Litomosoides carinii brought about adherence and cytotoxicity of normal rat spleen and peritoneal cells to microfilariae. The activity could be absorbed to, and eluted from, anti-rat IgE, but not anti-rat IgG, immunoabsorbent columns. Immune serum heated to 56°C for 3 hr did not cause cellular adherence or cytotoxicity; the addition of fresh normal rat serum failed to restore activity. Fresh rat serum did, however, restore activity to immune serum which was inactive after being heated to 56°C for 30 min. EDTA, EGTA and diethylcarbamazine inhibited adherence. It is concluded that IgE antibodies are responsible for cellular adherence and cytotoxicity and that complement may play a part, as yet undefined, in these reactions.
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M3 - Article
C2 - 7438547
AN - SCOPUS:0018897914
SN - 0009-9104
VL - 41
SP - 107
EP - 114
JO - Clinical and Experimental Immunology
JF - Clinical and Experimental Immunology
IS - 1
ER -