Abstract
Transgenic constructs containing the murine L-myc gene under the control of the inununoglobulin transcriptional enhancer element (Eμ) are expressed at unexpectedly high levels in thymocytes and proliferating T cells compared with cells from bone marrow and proliferating B cells. In contrast, double transgenic animals bearing constructs containing the L- and N-myc genes similarly linked to the EH element maintain preferential L-myc expression in T cells but express the N-myc transgene preferentially in B cells. These results indicate that the L-myc gene contains elements that act in concert with the Eμ element to allow preferential expression in T lineage cells. In correspondence to the expression pattern, Eμ-L-myc transgenic mice show expanded thymic cortices and irregularly formed splenic follicles with expanded T cell areas. Moreover, the percentage of thymocytes positive for the surface marker 1C11, which defines thymic progenitor cells, activated T cells and preleukemic T cells, is dramatically raised in transgenic mice compared with normal littermates. Eμ-L-myc transgenic animals are predisposed to clonal lymphoid tumors, most of which are T cell lymphomas. The relative incidence, latency period, and degree of malignancy of Eμ -L-myc tumors compared with Eμ - N- or c-myc tumors is consistent with a lower oncogenic potential of the L-myc gene. However, the Eμ -L-myc tumors do not express detectable levels of endogenous myc family genes indicating that the L-myc protein can substitute for c- or N-myc in the generation and growth of lymphoid neoplasms.
Original language | English (US) |
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Pages (from-to) | 3659-3666 |
Number of pages | 8 |
Journal | EMBO Journal |
Volume | 9 |
Issue number | 11 |
State | Published - 1990 |
Externally published | Yes |
Keywords
- L-myc gene
- immunoglobulin heavy chain enhancer
- lymphoid tumors
- transgenic mice
ASJC Scopus subject areas
- General Neuroscience
- Molecular Biology
- General Biochemistry, Genetics and Molecular Biology
- General Immunology and Microbiology