IL-2-mediated upregulation of uPA and uPAR in natural killer cells

Gheath Al-Atrash; Yaming Xue; Richard P. Kitson; Ronald H. Goldfarb; Sreerama Shetty; Steven Idell; Prathap Kumar Shetty Halady

doi:10.1006/bbrc.2002.6627

IL-2-mediated upregulation of uPA and uPAR in natural killer cells

Gheath Al-Atrash, Yaming Xue, Richard P. Kitson, Ronald H. Goldfarb, Sreerama Shetty, Steven Idell, Prathap Kumar Shetty Halady

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Urokinase plasminogen activator (uPA) and its receptor uPAR play a major role in immune cell-mediated, including natural killer (NK) cell-mediated, degradation of extracellular matrices. Herein, we investigate the effects of IL-2 on NK cell uPA and uPAR. RNA and protein analyses showed upregulation of uPA and uPAR following IL-2 stimulation. Gel-shift assays and Western blots detected uPA and uPAR mRNA binding proteins (mRNABPs), previously shown to destabilize uPA and uPAR mRNA. Following IL-2 stimulation, a downregulation of uPAR mRNABP and a reciprocal induction of uPAR mRNA were noted. The increase in uPA following IL-2 stimulation appeared to be more transcriptionally regulated. These data suggest that IL-2 upregulates both uPA and uPAR in NK cells through posttranscriptional as well as transcriptional mechanisms, partially explaining increases in NK cell invasiveness following IL-2 stimulation.

Original language	English (US)
Pages (from-to)	184-189
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	292
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.2002.6627
State	Published - 2002
Externally published	Yes

Keywords

Cytokines
Gene regulation
Interleukin-2
NK cells
Urokinase

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "IL-2-mediated upregulation of uPA and uPAR in natural killer cells",

abstract = "Urokinase plasminogen activator (uPA) and its receptor uPAR play a major role in immune cell-mediated, including natural killer (NK) cell-mediated, degradation of extracellular matrices. Herein, we investigate the effects of IL-2 on NK cell uPA and uPAR. RNA and protein analyses showed upregulation of uPA and uPAR following IL-2 stimulation. Gel-shift assays and Western blots detected uPA and uPAR mRNA binding proteins (mRNABPs), previously shown to destabilize uPA and uPAR mRNA. Following IL-2 stimulation, a downregulation of uPAR mRNABP and a reciprocal induction of uPAR mRNA were noted. The increase in uPA following IL-2 stimulation appeared to be more transcriptionally regulated. These data suggest that IL-2 upregulates both uPA and uPAR in NK cells through posttranscriptional as well as transcriptional mechanisms, partially explaining increases in NK cell invasiveness following IL-2 stimulation.",

keywords = "Cytokines, Gene regulation, Interleukin-2, NK cells, Urokinase",

author = "Gheath Al-Atrash and Yaming Xue and Kitson, {Richard P.} and Goldfarb, {Ronald H.} and Sreerama Shetty and Steven Idell and Halady, {Prathap Kumar Shetty}",

note = "Funding Information: We thank Ms. Kathy Johnson for her technical assistance. This work was supported in part by NIH HL 62453 (SS) and HL 45018 (SI) and Grant 000130-0080-1999 from the Texas Higher Education Coordinating Board Advanced Technology Program.",

year = "2002",

doi = "10.1006/bbrc.2002.6627",

language = "English (US)",

volume = "292",

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AU - Al-Atrash, Gheath

AU - Xue, Yaming

AU - Kitson, Richard P.

AU - Goldfarb, Ronald H.

AU - Shetty, Sreerama

AU - Idell, Steven

AU - Halady, Prathap Kumar Shetty

N1 - Funding Information: We thank Ms. Kathy Johnson for her technical assistance. This work was supported in part by NIH HL 62453 (SS) and HL 45018 (SI) and Grant 000130-0080-1999 from the Texas Higher Education Coordinating Board Advanced Technology Program.

PY - 2002

Y1 - 2002

N2 - Urokinase plasminogen activator (uPA) and its receptor uPAR play a major role in immune cell-mediated, including natural killer (NK) cell-mediated, degradation of extracellular matrices. Herein, we investigate the effects of IL-2 on NK cell uPA and uPAR. RNA and protein analyses showed upregulation of uPA and uPAR following IL-2 stimulation. Gel-shift assays and Western blots detected uPA and uPAR mRNA binding proteins (mRNABPs), previously shown to destabilize uPA and uPAR mRNA. Following IL-2 stimulation, a downregulation of uPAR mRNABP and a reciprocal induction of uPAR mRNA were noted. The increase in uPA following IL-2 stimulation appeared to be more transcriptionally regulated. These data suggest that IL-2 upregulates both uPA and uPAR in NK cells through posttranscriptional as well as transcriptional mechanisms, partially explaining increases in NK cell invasiveness following IL-2 stimulation.

AB - Urokinase plasminogen activator (uPA) and its receptor uPAR play a major role in immune cell-mediated, including natural killer (NK) cell-mediated, degradation of extracellular matrices. Herein, we investigate the effects of IL-2 on NK cell uPA and uPAR. RNA and protein analyses showed upregulation of uPA and uPAR following IL-2 stimulation. Gel-shift assays and Western blots detected uPA and uPAR mRNA binding proteins (mRNABPs), previously shown to destabilize uPA and uPAR mRNA. Following IL-2 stimulation, a downregulation of uPAR mRNABP and a reciprocal induction of uPAR mRNA were noted. The increase in uPA following IL-2 stimulation appeared to be more transcriptionally regulated. These data suggest that IL-2 upregulates both uPA and uPAR in NK cells through posttranscriptional as well as transcriptional mechanisms, partially explaining increases in NK cell invasiveness following IL-2 stimulation.

KW - Cytokines

KW - Gene regulation

KW - Interleukin-2

KW - NK cells

KW - Urokinase

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IL-2-mediated upregulation of uPA and uPAR in natural killer cells

Abstract

Keywords

ASJC Scopus subject areas

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