@inproceedings{0d6d9676041c4ef7b3ff76fb57ba9790,
title = "Imaging mechanical properties of cancer cells during metastasis with Brillouin microspectroscopy",
abstract = "Cancer remains among the leading causes of death in the United States. Early detection, classification and understanding of malignant cell proliferation and metastasis mechanisms are crucial for effective treatment. Current malignant cell studies largely rely on either invasive imaging techniques or invasive research protocols that hinder both speed and accuracy of cancer research. Here we are reporting successful imaging of cancer metastasis processes on a cellular level using Brillouin microspectroscopic imaging. In this research we are specifically presenting results of a non-invasive interrogation of elastic properties of 4T1 murine fibroblast cells in a spheroid model acquired with our custom-built confocal Brillouin microspectrometer. Spatial map of elastic properties was recorded for both interior and exterior regions of the 4T1 cell spheroid. We observed lower stiffness of cancer cells compared to cells from internal regions. In addition we observed the difference in stiffness values between cells exposed to challenging and normal environmental conditions. Our findings correlate well with prior published data, acquired with conventional biomechanical assessment techniques.",
keywords = "Brillouin Spectroscopy, Cancer cell, Cell proliferation, Confocal microscopy, Elastic properties, Fibroblast, Image processing, Imaging spectroscopy, Metastasis, Multimodal imaging, Raman, Scattering",
author = "Vsevolod Cheburkanov and Kavya Pendyala and Maria Parlani and Tanmay Lele and Peter Friedl and Vladislav Yakovlev",
note = "Funding Information: This work was partially supported by funding from NSF (DBI-1455671, ECCS-1509268, CMMI-1826078), AFOSR (FA9550-15-1-0517, FA9550-20-1-0366, FA9550-20-1-0367), DOD Army Medical Research (W81XWH2010777), NIH (1R01GM127696-01, 1R21GM142107-01), and the Cancer Prevention and Research Institute of Texas (RP180588). Publisher Copyright: {\textcopyright} 2022 SPIE.; Multiscale Imaging and Spectroscopy III 2022 ; Conference date: 20-02-2022 Through 24-02-2022",
year = "2022",
doi = "10.1117/12.2610539",
language = "English (US)",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Campagnola, {Paul J.} and Maitland, {Kristen C.} and Roblyer, {Darren M.}",
booktitle = "Multiscale Imaging and Spectroscopy III",
}