Immunocamouflage of latex surfaces by grafted methoxypoly(ethylene glycol) (mPEG): Proteomic analysis of plasma protein adsorption

Yevgeniya Le; Li Li; Dun Cheng Wang; Mark D. Scott

doi:10.1007/s11427-012-4290-2

Immunocamouflage of latex surfaces by grafted methoxypoly(ethylene glycol) (mPEG): Proteomic analysis of plasma protein adsorption

Yevgeniya Le, Li Li, Dun Cheng Wang, Mark D. Scott

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Grafting of methoxypoly(ethylene glycol) (mPEG) to cells and biomaterials is a promising non-pharmacological immunomodulation technology. However, due to the labile nature of cells, surface-plasma interactions are poorly understood; hence, a latex bead model was studied. PEGylation of beads resulted in a density and molecular weight dependent decrease in total adsorbed protein with a net reduction from (159. 9±6. 4) ng cm ^-2 on bare latex to (18. 4±0. 8) and (52. 3±5. 3) ng cm ^-2 on PEGylated beads (1 mmol L ^-1 of 2 or 20 kD SCmPEG, respectively). SDS-PAGE and iTRAQ-MS analysis revealed differential compositions of the adsorbed protein layer on the PEGylated latex with a significant reduction in the compositional abundance of proteins involved in immune system activation. Thus, the biological efficacy of immunocamouflaged cells and materials is mediated by both biophysical obfuscation of antigens and reduced surface-macromolecule interactions.

Original language	English (US)
Pages (from-to)	191-201
Number of pages	11
Journal	Science China Life Sciences
Volume	55
Issue number	3
DOIs	https://doi.org/10.1007/s11427-012-4290-2
State	Published - Mar 2012
Externally published	Yes

Keywords

immunocamouflage
iTRAQ
mass spectrometry
methoxypoly(ethylene glycol)
PEGylation
polymer
polystyrene latex
protein adsorption
proteomics

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Environmental Science
General Agricultural and Biological Sciences

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10.1007/s11427-012-4290-2

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@article{6bb3278ae00d4a599266a2618f7e9dc5,

title = "Immunocamouflage of latex surfaces by grafted methoxypoly(ethylene glycol) (mPEG): Proteomic analysis of plasma protein adsorption",

abstract = "Grafting of methoxypoly(ethylene glycol) (mPEG) to cells and biomaterials is a promising non-pharmacological immunomodulation technology. However, due to the labile nature of cells, surface-plasma interactions are poorly understood; hence, a latex bead model was studied. PEGylation of beads resulted in a density and molecular weight dependent decrease in total adsorbed protein with a net reduction from (159. 9±6. 4) ng cm -2 on bare latex to (18. 4±0. 8) and (52. 3±5. 3) ng cm -2 on PEGylated beads (1 mmol L -1 of 2 or 20 kD SCmPEG, respectively). SDS-PAGE and iTRAQ-MS analysis revealed differential compositions of the adsorbed protein layer on the PEGylated latex with a significant reduction in the compositional abundance of proteins involved in immune system activation. Thus, the biological efficacy of immunocamouflaged cells and materials is mediated by both biophysical obfuscation of antigens and reduced surface-macromolecule interactions.",

keywords = "immunocamouflage, iTRAQ, mass spectrometry, methoxypoly(ethylene glycol), PEGylation, polymer, polystyrene latex, protein adsorption, proteomics",

author = "Yevgeniya Le and Li Li and Wang, {Dun Cheng} and Scott, {Mark D.}",

note = "Funding Information: The authors thank Drs. Peter Schubert (Canadian Blood Services and University of British Columbia, Vancouver, BC, Canada) and Jurgen Kast (University of British Columbia, Biomedical Research Centre, Vancouver, BC, Canada) for their helpful comments in the manuscript preparation. In addition, we acknowledge Mr. Derek Smith (University of Victoria, Genome BC Proteomics Centre, Victoria, BC, Canada) for his technical assistance and expertise and Dr. Geraldine Walsh, CBS Scientific Writer, for writing and editorial assistance. This work was supported by grants from the Canadian Blood Services, Canadian Blood Services-Canadian Institutes of Health Research (CBS-CIHR) Partnership Fund and Health Canada. The views expressed herein do not necessarily represent the view of the federal government of Canada. We thank the Canada Foundation for Innovation and the Michael Smith Foundation for Health Research for infrastructure funding at the University of British Columbia Centre for Blood Research.",

year = "2012",

month = mar,

doi = "10.1007/s11427-012-4290-2",

language = "English (US)",

volume = "55",

pages = "191--201",

journal = "Science China Life Sciences",

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T2 - Proteomic analysis of plasma protein adsorption

AU - Le, Yevgeniya

AU - Li, Li

AU - Wang, Dun Cheng

AU - Scott, Mark D.

N1 - Funding Information: The authors thank Drs. Peter Schubert (Canadian Blood Services and University of British Columbia, Vancouver, BC, Canada) and Jurgen Kast (University of British Columbia, Biomedical Research Centre, Vancouver, BC, Canada) for their helpful comments in the manuscript preparation. In addition, we acknowledge Mr. Derek Smith (University of Victoria, Genome BC Proteomics Centre, Victoria, BC, Canada) for his technical assistance and expertise and Dr. Geraldine Walsh, CBS Scientific Writer, for writing and editorial assistance. This work was supported by grants from the Canadian Blood Services, Canadian Blood Services-Canadian Institutes of Health Research (CBS-CIHR) Partnership Fund and Health Canada. The views expressed herein do not necessarily represent the view of the federal government of Canada. We thank the Canada Foundation for Innovation and the Michael Smith Foundation for Health Research for infrastructure funding at the University of British Columbia Centre for Blood Research.

PY - 2012/3

Y1 - 2012/3

N2 - Grafting of methoxypoly(ethylene glycol) (mPEG) to cells and biomaterials is a promising non-pharmacological immunomodulation technology. However, due to the labile nature of cells, surface-plasma interactions are poorly understood; hence, a latex bead model was studied. PEGylation of beads resulted in a density and molecular weight dependent decrease in total adsorbed protein with a net reduction from (159. 9±6. 4) ng cm -2 on bare latex to (18. 4±0. 8) and (52. 3±5. 3) ng cm -2 on PEGylated beads (1 mmol L -1 of 2 or 20 kD SCmPEG, respectively). SDS-PAGE and iTRAQ-MS analysis revealed differential compositions of the adsorbed protein layer on the PEGylated latex with a significant reduction in the compositional abundance of proteins involved in immune system activation. Thus, the biological efficacy of immunocamouflaged cells and materials is mediated by both biophysical obfuscation of antigens and reduced surface-macromolecule interactions.

AB - Grafting of methoxypoly(ethylene glycol) (mPEG) to cells and biomaterials is a promising non-pharmacological immunomodulation technology. However, due to the labile nature of cells, surface-plasma interactions are poorly understood; hence, a latex bead model was studied. PEGylation of beads resulted in a density and molecular weight dependent decrease in total adsorbed protein with a net reduction from (159. 9±6. 4) ng cm -2 on bare latex to (18. 4±0. 8) and (52. 3±5. 3) ng cm -2 on PEGylated beads (1 mmol L -1 of 2 or 20 kD SCmPEG, respectively). SDS-PAGE and iTRAQ-MS analysis revealed differential compositions of the adsorbed protein layer on the PEGylated latex with a significant reduction in the compositional abundance of proteins involved in immune system activation. Thus, the biological efficacy of immunocamouflaged cells and materials is mediated by both biophysical obfuscation of antigens and reduced surface-macromolecule interactions.

KW - immunocamouflage

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KW - mass spectrometry

KW - methoxypoly(ethylene glycol)

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KW - proteomics

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ER -

Immunocamouflage of latex surfaces by grafted methoxypoly(ethylene glycol) (mPEG): Proteomic analysis of plasma protein adsorption

Abstract

Keywords

ASJC Scopus subject areas

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