TY - JOUR
T1 - Immunocytochemistry of serous effusion specimens
T2 - A comparison of ThinPrep® vs. cell block
AU - Gong, Yun
AU - Sun, Xiaoping
AU - Michael, Claire W.
AU - Attal, Sam
AU - Williamson, Beth A.
AU - Bedrossian, Carlos W.M.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2003/1/1
Y1 - 2003/1/1
N2 - The ThinPrep® Processor is gaining popularity in both gynecologic and nongynecologic cytologic samples, including effusion specimens. We compared immunocytochemical results on Thin-Prep and cell-block preparations from the same effusion specimen with antibodies commonly used in effusion cytopathology. Samples from 17 reactive effusions and 79 effusions with metastatic adenocarcinomas were each prepared as monolayer ThinPreps and formalin-fixed, paraffin-embedded cell blocks. All slides were immunostained with antibodies against intermediate filaments (cytokeratins 8 and 18, and vimentin), cytoplasmic or membrane proteins (EMA, polyclonal CEA, B72.3, and BerEP4), and nuclear antigens (Ki67, PCNA, and p53), using the avidin-biotin procedure. ThinPrep and cell-block slides were stained simultaneously, using identical protocols. Both preparations showed similar results with respect to frequency of positivity, intensity, and distribution of stain for all nonnuclear markers tested. However, for the three nuclear markers Ki67, PCNA, and p53, the frequency and intensity of reaction with ThinPrep were significantly lower than with the cell-block preparation, particularly in malignant effusions. These findings suggest that immunocytochemical results obtained with ThinPrep® match those of cell block for most markers tested. However, cell-block preparation are superior to Thin-Prep for nuclear markers (Ki67, PCNA, and p53).
AB - The ThinPrep® Processor is gaining popularity in both gynecologic and nongynecologic cytologic samples, including effusion specimens. We compared immunocytochemical results on Thin-Prep and cell-block preparations from the same effusion specimen with antibodies commonly used in effusion cytopathology. Samples from 17 reactive effusions and 79 effusions with metastatic adenocarcinomas were each prepared as monolayer ThinPreps and formalin-fixed, paraffin-embedded cell blocks. All slides were immunostained with antibodies against intermediate filaments (cytokeratins 8 and 18, and vimentin), cytoplasmic or membrane proteins (EMA, polyclonal CEA, B72.3, and BerEP4), and nuclear antigens (Ki67, PCNA, and p53), using the avidin-biotin procedure. ThinPrep and cell-block slides were stained simultaneously, using identical protocols. Both preparations showed similar results with respect to frequency of positivity, intensity, and distribution of stain for all nonnuclear markers tested. However, for the three nuclear markers Ki67, PCNA, and p53, the frequency and intensity of reaction with ThinPrep were significantly lower than with the cell-block preparation, particularly in malignant effusions. These findings suggest that immunocytochemical results obtained with ThinPrep® match those of cell block for most markers tested. However, cell-block preparation are superior to Thin-Prep for nuclear markers (Ki67, PCNA, and p53).
KW - Alcohol
KW - Body effusions
KW - Cell block
KW - Cytology
KW - Formalin
KW - Immunocytochemistry
KW - Nuclear markers
KW - ThinPrep
UR - http://www.scopus.com/inward/record.url?scp=0037222737&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037222737&partnerID=8YFLogxK
U2 - 10.1002/dc.10219
DO - 10.1002/dc.10219
M3 - Article
C2 - 12508174
AN - SCOPUS:0037222737
SN - 8755-1039
VL - 28
SP - 1
EP - 5
JO - Diagnostic Cytopathology
JF - Diagnostic Cytopathology
IS - 1
ER -