Immunogenicity of HLA antigens purified from serum

Soldano Ferrone; Kamal K. Mittal; Michele A. Pellegrino; James P. Allison; Ralph A. Reisfeld

doi:10.1097/00007890-197701000-00002

Immunogenicity of HLA antigens purified from serum

Soldano Ferrone, Kamal K. Mittal, Michele A. Pellegrino, James P. Allison, Ralph A. Reisfeld

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

The immunogenic properties of HLA-A9 antigens isolated from serum have been evaluated. A9 antigens at various stages of purification can elicit the formation of cytotoxic antibodies which become operationally specific to A9 either after absorption of the xenoantisera with cultured human lymphoid cells or human red blood cells, or after dilution of xenoantisera with human serum. A9 xenoantisera do not affect mixed lymphocyte reactions between allogeneic lymphocytes carrying A9, suggesting that coating of antigens of the A locus does not impair the functional activity of lymphocytes in the mixed lymphocyte reaction.

Original language	English (US)
Pages (from-to)	7-15
Number of pages	9
Journal	Transplantation
Volume	23
Issue number	1
DOIs	https://doi.org/10.1097/00007890-197701000-00002
State	Published - Jan 1977
Externally published	Yes

ASJC Scopus subject areas

Transplantation

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10.1097/00007890-197701000-00002

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title = "Immunogenicity of HLA antigens purified from serum",

abstract = "The immunogenic properties of HLA-A9 antigens isolated from serum have been evaluated. A9 antigens at various stages of purification can elicit the formation of cytotoxic antibodies which become operationally specific to A9 either after absorption of the xenoantisera with cultured human lymphoid cells or human red blood cells, or after dilution of xenoantisera with human serum. A9 xenoantisera do not affect mixed lymphocyte reactions between allogeneic lymphocytes carrying A9, suggesting that coating of antigens of the A locus does not impair the functional activity of lymphocytes in the mixed lymphocyte reaction.",

author = "Soldano Ferrone and Mittal, {Kamal K.} and Pellegrino, {Michele A.} and Allison, {James P.} and Reisfeld, {Ralph A.}",

year = "1977",

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doi = "10.1097/00007890-197701000-00002",

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AU - Ferrone, Soldano

AU - Mittal, Kamal K.

AU - Pellegrino, Michele A.

AU - Allison, James P.

AU - Reisfeld, Ralph A.

PY - 1977/1

Y1 - 1977/1

N2 - The immunogenic properties of HLA-A9 antigens isolated from serum have been evaluated. A9 antigens at various stages of purification can elicit the formation of cytotoxic antibodies which become operationally specific to A9 either after absorption of the xenoantisera with cultured human lymphoid cells or human red blood cells, or after dilution of xenoantisera with human serum. A9 xenoantisera do not affect mixed lymphocyte reactions between allogeneic lymphocytes carrying A9, suggesting that coating of antigens of the A locus does not impair the functional activity of lymphocytes in the mixed lymphocyte reaction.

AB - The immunogenic properties of HLA-A9 antigens isolated from serum have been evaluated. A9 antigens at various stages of purification can elicit the formation of cytotoxic antibodies which become operationally specific to A9 either after absorption of the xenoantisera with cultured human lymphoid cells or human red blood cells, or after dilution of xenoantisera with human serum. A9 xenoantisera do not affect mixed lymphocyte reactions between allogeneic lymphocytes carrying A9, suggesting that coating of antigens of the A locus does not impair the functional activity of lymphocytes in the mixed lymphocyte reaction.

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Immunogenicity of HLA antigens purified from serum

Abstract

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