TY - JOUR
T1 - Immunogenicity of neutralizing epitopes on multiple-epitope vaccines against HIV-1
AU - Lu, Yun
AU - Xiao, Yi
AU - Ding, Jian
AU - Dierich, Manfred P.
AU - Chen, Ying Hua
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2000
Y1 - 2000
N2 - Based on our hypothesis that epitope vaccine may be a new strategy to induce high levels of neutralization antibodies against HIV-1, we prepared multiple-epitope vaccines using three neutralizing epitopes (GPGRAFY, RILAVERYLKD and ELDKWA) of HIV-1 gp160, and characterized their immunogenicity. Peptide 1 (C-G-(ELDKWA-GPGRAFY)2-K] and peptide 2 (CG-GPGRAFY-ELDKWA-G-RILAVERYLKD) were synthesized and conjugated with carrier protein bovine serum albumin (BSA). After vaccination antibody responses to these immunogens were induced and evaluated by ELISA. The C-G-(ELDKWA-GPGRAFY)2-K-BSA (BSA: carrier protein) multiple-epitope vaccine induced a strong antibody response to the C-G-(ELDKWA-GPGRAFY)2-K peptide (antibody titer: 1:25,600) and C-(ELDKWAG)4 peptide (antibody titer: 1:12,800), but a weak antibody response to the C-(GPCGRAFY)4 peptide. The CG-GP-GRAFY-ELDKWA-G-RILAVERYLKD-K-BSA (BSA: carrier protein) multiple-epitope vaccine also induced strong antibody response to the CG-GPGRAFY-ELDKWA-G-RILAVERYLKD-K peptide (antibody titer: 1:25,600) and C-(ELLDKWAG)4 peptide (antibody titer: 1:6,400), a very strong response to C-(RIVALVERYLKD-G)2-K peptide (dilution: 1:102,400), and a very weak response to the C(GPGRAFY)4 peptide (dilution: 1:400) in mice. Both antisera induced by both multiple-epitope vaccines interacted with the recombinant soluble gp41 (rgp41), but did not bind two control peptides. In comparison with both epitope vaccines, the rgp160 subunit vaccine could induce weak epitope-specific antibody response to these three epitopes on the three epitope peptides and V3, N-domain and C-domain peptides (dilution: 1:400-1:1,600). These results indicate that both multiple-epitope vaccines could induce high levels of antibodies to both neutralizing epitopes RILAVERYLKD and ELDKWA, while the GPGRAFY epitope on both vaccines appeared to have weak immunogenicity. Both multiple-epitope vaccines showed significant potency on inducing high levels of epitope-specific neutralization antibodies in comparison with rgp160 subunit vaccine. Copyright (C) 2000 S. Karger AG, Basel.
AB - Based on our hypothesis that epitope vaccine may be a new strategy to induce high levels of neutralization antibodies against HIV-1, we prepared multiple-epitope vaccines using three neutralizing epitopes (GPGRAFY, RILAVERYLKD and ELDKWA) of HIV-1 gp160, and characterized their immunogenicity. Peptide 1 (C-G-(ELDKWA-GPGRAFY)2-K] and peptide 2 (CG-GPGRAFY-ELDKWA-G-RILAVERYLKD) were synthesized and conjugated with carrier protein bovine serum albumin (BSA). After vaccination antibody responses to these immunogens were induced and evaluated by ELISA. The C-G-(ELDKWA-GPGRAFY)2-K-BSA (BSA: carrier protein) multiple-epitope vaccine induced a strong antibody response to the C-G-(ELDKWA-GPGRAFY)2-K peptide (antibody titer: 1:25,600) and C-(ELDKWAG)4 peptide (antibody titer: 1:12,800), but a weak antibody response to the C-(GPCGRAFY)4 peptide. The CG-GP-GRAFY-ELDKWA-G-RILAVERYLKD-K-BSA (BSA: carrier protein) multiple-epitope vaccine also induced strong antibody response to the CG-GPGRAFY-ELDKWA-G-RILAVERYLKD-K peptide (antibody titer: 1:25,600) and C-(ELLDKWAG)4 peptide (antibody titer: 1:6,400), a very strong response to C-(RIVALVERYLKD-G)2-K peptide (dilution: 1:102,400), and a very weak response to the C(GPGRAFY)4 peptide (dilution: 1:400) in mice. Both antisera induced by both multiple-epitope vaccines interacted with the recombinant soluble gp41 (rgp41), but did not bind two control peptides. In comparison with both epitope vaccines, the rgp160 subunit vaccine could induce weak epitope-specific antibody response to these three epitopes on the three epitope peptides and V3, N-domain and C-domain peptides (dilution: 1:400-1:1,600). These results indicate that both multiple-epitope vaccines could induce high levels of antibodies to both neutralizing epitopes RILAVERYLKD and ELDKWA, while the GPGRAFY epitope on both vaccines appeared to have weak immunogenicity. Both multiple-epitope vaccines showed significant potency on inducing high levels of epitope-specific neutralization antibodies in comparison with rgp160 subunit vaccine. Copyright (C) 2000 S. Karger AG, Basel.
KW - HIV-1
KW - Immunogenicity
KW - Multiple-epitope vaccine
KW - Neutralizing epitope
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U2 - 10.1159/000024300
DO - 10.1159/000024300
M3 - Article
C2 - 10686512
AN - SCOPUS:0033981135
SN - 1018-2438
VL - 121
SP - 80
EP - 84
JO - International Archives of Allergy and Immunology
JF - International Archives of Allergy and Immunology
IS - 1
ER -