TY - JOUR
T1 - Immunohistochemical features of the pseudomyxoma peritonei microenvironment
T2 - An opportunity for clinicians
AU - Grizzi, Fabio
AU - Cananzi, Ferdinando Carlo Maria
AU - Battista, Serena
AU - Brambilla, Tatiana
AU - Qehajaj, Dorina
AU - Chiriva-Internati, Maurizio
AU - Romario, Uberto Fumagalli
AU - Quagliuolo, Vittorio
AU - Bagnoli, Pietro Francesco
N1 - Publisher Copyright:
© Science Printers and Publishers, Inc.
PY - 2018/6/1
Y1 - 2018/6/1
N2 - OBJECTIVE: To provide new details on the pseudomyxoma peritonei (PMP) microenvironment and discuss its potential role on the clinical behavior of this tumor. STUDY DESIGN: In the present study, in addition to routine histology, immunostains for CD68, CD3, CD20, Ki-67, CD34, LYVE-1, and the tumor-associated antigens (TAAs) pituitary-tumor transforming gene 1 (PTTG1) and squamous cell carcinoma antigen 1 (SCCA1) were explored. Fourteen consecutive patients who underwent cytoreductive surgery with hyperthermic intraoperative peritoneal chemotherapy were included in the study. RESULTS: We found the presence of variable amounts and pattern distributions of CD68+ cells, CD3+ T-cells, and CD20+ B-cells in the PMP microenvironment. CD3+ lymphocytes were grouped in clusters in 7 out of 14 (50%) PMPs, while only 4 out of 14 (29%) had dispersed CD20+ lymphocytes. CD68+ macrophages were always found dispersed throughout the PMP microenvironment. PMPs have been also found highly vascularized by blood vessels when compared to LYVE-1+ lymphatic vessels, and variably proliferative as shown by different Ki- 67+ cell densities. Additionally, PMPs were immunopositive for PTTG1 and SCCA1, 2 TAAs previously associated with the progression and recurrence of various human malignancies. CONCLUSION: Our findings highlight unknown features about PMP microenvironment organization and represent a basis for additional studies including a large cohort of patients to reveal helpful information on the clinical behavior of this tumor.
AB - OBJECTIVE: To provide new details on the pseudomyxoma peritonei (PMP) microenvironment and discuss its potential role on the clinical behavior of this tumor. STUDY DESIGN: In the present study, in addition to routine histology, immunostains for CD68, CD3, CD20, Ki-67, CD34, LYVE-1, and the tumor-associated antigens (TAAs) pituitary-tumor transforming gene 1 (PTTG1) and squamous cell carcinoma antigen 1 (SCCA1) were explored. Fourteen consecutive patients who underwent cytoreductive surgery with hyperthermic intraoperative peritoneal chemotherapy were included in the study. RESULTS: We found the presence of variable amounts and pattern distributions of CD68+ cells, CD3+ T-cells, and CD20+ B-cells in the PMP microenvironment. CD3+ lymphocytes were grouped in clusters in 7 out of 14 (50%) PMPs, while only 4 out of 14 (29%) had dispersed CD20+ lymphocytes. CD68+ macrophages were always found dispersed throughout the PMP microenvironment. PMPs have been also found highly vascularized by blood vessels when compared to LYVE-1+ lymphatic vessels, and variably proliferative as shown by different Ki- 67+ cell densities. Additionally, PMPs were immunopositive for PTTG1 and SCCA1, 2 TAAs previously associated with the progression and recurrence of various human malignancies. CONCLUSION: Our findings highlight unknown features about PMP microenvironment organization and represent a basis for additional studies including a large cohort of patients to reveal helpful information on the clinical behavior of this tumor.
KW - Biomarkers
KW - Immunity
KW - Pituitary-tumor transforming gene 1
KW - Pseudomyxoma peritonei
KW - Squamous cell carcinoma antigen 1
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M3 - Article
AN - SCOPUS:85049805879
SN - 2578-742X
VL - 40
SP - 109
EP - 115
JO - Analytical and Quantitative Cytopathology and Histopathology
JF - Analytical and Quantitative Cytopathology and Histopathology
IS - 3
ER -