Abstract
Tie present studies are concerned with the immunologic specificity and reactivity of 2 distinct antimacrophage sera (AMS). Anti-mouse and anti-guinea pig macrophage sera were obtained by immunizing goats with highly purified populations of either mouse or guinea pig macrophges. The antisera were absorbed extensively with several normal and neoplastic cells before specific antimacrophage activity could be demonstrated. We report the results of reciprocal experiments that used the 2 separate AMS, which were tested against 2 respective and distinct macrophage populations. The absorbed AMS bound specifically to and also lysed their respective macrophages in the presence of complement. The lytic effects of AMS treatment in vitro were cumulative: thus, 2 consecutive treatments with these antisera produced the greatest cytotoxic effect on the macrophages. Goat anti-mouse macrophage serum neither bound to nor lysed guinea pig macrophages; goat anti-guinea pig macrophage serum neither bound to nor lysed mouse macrophages. Thus, no cross-reactivity between mouse and guinea pig macrophages could be demonstrated.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 119-130 |
| Number of pages | 12 |
| Journal | RES Journal of the Reticuloendothelial Society |
| Volume | 21 |
| Issue number | 2 |
| State | Published - 1977 |
| Externally published | Yes |
ASJC Scopus subject areas
- Hematology