TY - JOUR
T1 - Immunostaining for rapid diagnosis of acute promyelocytic leukemia with the tetramethylrhodamine- 5-isothiocyanate-conjugated anti-promyelocytic leukemia monoclonal antibody PG-M3
AU - Alayed Dr., Khaled M.
AU - Medeiros Dr., L. Jeffrey
AU - Phan Dr., Danh
AU - Ojiaku Dr., Chinemerem C.
AU - Patel Dr., Jyoti
AU - Yap Dr., Jan Paul V.
AU - McCord Dr., Yen
AU - Woods Dr., Janaya S.
AU - Konoplev Dr., Sergej
AU - Bueso-Ramos Dr., Carlos E.
AU - Reyes Dr., Steven R.
PY - 2013/11
Y1 - 2013/11
N2 - Context.-Anti-promyelocytic leukemia (PML) immunofluorescence staining is a known diagnostic tool for rapid diagnosis of acute promyelocytic leukemia (APL). Objective.-We describe our methods using the recently developed, commercially available, tetramethylrhodamine- 5-isothiocyanate-labeled PG-M3 anti-PML antibody for APL testing. Design.-Immunofluorescence staining with the tetramethylrhodamine- 5-isothiocyanate-labeled PG-M3 antibody was used to detect PML-RARA in bone marrow aspirate and/or peripheral blood smears from 30 patients with acute leukemia. The results were compared with those of concurrent testing with our in-house polyclonal anti-PML antibody and with established tests. Results.-All APL cases showed a positive (fine/microgranular) immunofluorescence staining pattern, whereas non-APL cases showed a negative (chunky/macrogranular) pattern. These results, which were available within 2 hours, were validated by testing with the polyclonal anti- PML antibody and with established cytogenetic and molecular testing methods. Conclusions.-We validated the utility of the tetramethylrhodamine-5-isothiocyanate-labeled anti-PML antibody PG-M3 for the diagnosis of APL. Our results indicate that immunofluorescence staining with this antibody is a rapid and reliable method for the diagnosis of APL.
AB - Context.-Anti-promyelocytic leukemia (PML) immunofluorescence staining is a known diagnostic tool for rapid diagnosis of acute promyelocytic leukemia (APL). Objective.-We describe our methods using the recently developed, commercially available, tetramethylrhodamine- 5-isothiocyanate-labeled PG-M3 anti-PML antibody for APL testing. Design.-Immunofluorescence staining with the tetramethylrhodamine- 5-isothiocyanate-labeled PG-M3 antibody was used to detect PML-RARA in bone marrow aspirate and/or peripheral blood smears from 30 patients with acute leukemia. The results were compared with those of concurrent testing with our in-house polyclonal anti-PML antibody and with established tests. Results.-All APL cases showed a positive (fine/microgranular) immunofluorescence staining pattern, whereas non-APL cases showed a negative (chunky/macrogranular) pattern. These results, which were available within 2 hours, were validated by testing with the polyclonal anti- PML antibody and with established cytogenetic and molecular testing methods. Conclusions.-We validated the utility of the tetramethylrhodamine-5-isothiocyanate-labeled anti-PML antibody PG-M3 for the diagnosis of APL. Our results indicate that immunofluorescence staining with this antibody is a rapid and reliable method for the diagnosis of APL.
UR - http://www.scopus.com/inward/record.url?scp=84887833833&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84887833833&partnerID=8YFLogxK
U2 - 10.5858/arpa.2012-0565-OA
DO - 10.5858/arpa.2012-0565-OA
M3 - Article
C2 - 24168508
AN - SCOPUS:84887833833
SN - 0003-9985
VL - 137
SP - 1669
EP - 1673
JO - Archives of Pathology and Laboratory Medicine
JF - Archives of Pathology and Laboratory Medicine
IS - 11
ER -