TY - JOUR
T1 - Implementation and performance of SIBYLS
T2 - A dual endstation small-angle X-ray scattering and macromolecular crystallography beamline at the Advanced Light Source
AU - Classen, Scott
AU - Hura, Greg L.
AU - Holton, James M.
AU - Rambo, Robert P.
AU - Rodic, Ivan
AU - McGuire, Patrick J.
AU - Dyer, Kevin
AU - Hammel, Michal
AU - Meigs, George
AU - Frankel, Kenneth A.
AU - Tainer, John A.
PY - 2013/2
Y1 - 2013/2
N2 - The SIBYLS beamline (12.3.1) of the Advanced Light Source at Lawrence Berkeley National Laboratory, supported by the US Department of Energy and the National Institutes of Health, is optimized for both small-angle X-ray scattering (SAXS) and macromolecular crystallography (MX), making it unique among the world's mostly SAXS or MX dedicated beamlines. Since SIBYLS was commissioned, assessments of the limitations and advantages of a combined SAXS and MX beamline have suggested new strategies for integration and optimal data collection methods and have led to additional hardware and software enhancements. Features described include a dual mode monochromator [containing both Si(111) crystals and Mo/B4C multilayer elements], rapid beamline optics conversion between SAXS and MX modes, active beam stabilization, sample-loading robotics, and mail-in and remote data collection. These features allow users to gain valuable insights from both dynamic solution scattering and high-resolution atomic diffraction experiments performed at a single synchrotron beamline. Key practical issues considered for data collection and analysis include radiation damage, structural ensembles, alternative conformers and flexibility. SIBYLS develops and applies efficient combined MX and SAXS methods that deliver high-impact results by providing robust cost-effective routes to connect structures to biology and by performing experiments that aid beamline designs for next generation light sources.
AB - The SIBYLS beamline (12.3.1) of the Advanced Light Source at Lawrence Berkeley National Laboratory, supported by the US Department of Energy and the National Institutes of Health, is optimized for both small-angle X-ray scattering (SAXS) and macromolecular crystallography (MX), making it unique among the world's mostly SAXS or MX dedicated beamlines. Since SIBYLS was commissioned, assessments of the limitations and advantages of a combined SAXS and MX beamline have suggested new strategies for integration and optimal data collection methods and have led to additional hardware and software enhancements. Features described include a dual mode monochromator [containing both Si(111) crystals and Mo/B4C multilayer elements], rapid beamline optics conversion between SAXS and MX modes, active beam stabilization, sample-loading robotics, and mail-in and remote data collection. These features allow users to gain valuable insights from both dynamic solution scattering and high-resolution atomic diffraction experiments performed at a single synchrotron beamline. Key practical issues considered for data collection and analysis include radiation damage, structural ensembles, alternative conformers and flexibility. SIBYLS develops and applies efficient combined MX and SAXS methods that deliver high-impact results by providing robust cost-effective routes to connect structures to biology and by performing experiments that aid beamline designs for next generation light sources.
KW - SIBYLS
KW - macromolecular crystallography (MX)
KW - small-angle X-ray scattering (SAXS)
KW - synchrotron beamlines
UR - http://www.scopus.com/inward/record.url?scp=84872694541&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84872694541&partnerID=8YFLogxK
U2 - 10.1107/S0021889812048698
DO - 10.1107/S0021889812048698
M3 - Article
C2 - 23396808
AN - SCOPUS:84872694541
SN - 0021-8898
VL - 46
SP - 1
EP - 13
JO - Journal of Applied Crystallography
JF - Journal of Applied Crystallography
IS - 1
ER -