TY - JOUR
T1 - Improved cellular specificity of plasmonic nanobubbles versus nanoparticles in heterogeneous cell systems
AU - Lukianova-Hleb, Ekaterina Y.
AU - Ren, Xiaoyang
AU - Constantinou, Pamela E.
AU - Danysh, Brian P.
AU - Shenefelt, Derek L.
AU - Carson, Daniel D.
AU - Farach-Carson, Mary C.
AU - Kulchitsky, Vladimir A.
AU - Wu, Xiangwei
AU - Wagner, Daniel S.
AU - Lapotko, Dmitri O.
PY - 2012/4/3
Y1 - 2012/4/3
N2 - The limited specificity of nanoparticle (NP) uptake by target cells associated with a disease is one of the principal challenges of nanomedicine. Using the threshold mechanism of plasmonic nanobubble (PNB) generation and enhanced accumulation and clustering of gold nanoparticles in target cells, we increased the specificity of PNB generation and detection in target versus non-target cells by more than one order of magnitude compared to the specificity of NP uptake by the same cells. This improved cellular specificity of PNBs was demonstrated in six different cell models representing diverse molecular targets such as epidermal growth factor receptor, CD3 receptor, prostate specific membrane antigen and mucin molecule MUC1. Thus PNBs may be a universal method and nano-agent that overcome the problem of non-specific uptake of NPs by non-target cells and improve the specificity of NP-based diagnostics, therapeutics and theranostics at the cell level.
AB - The limited specificity of nanoparticle (NP) uptake by target cells associated with a disease is one of the principal challenges of nanomedicine. Using the threshold mechanism of plasmonic nanobubble (PNB) generation and enhanced accumulation and clustering of gold nanoparticles in target cells, we increased the specificity of PNB generation and detection in target versus non-target cells by more than one order of magnitude compared to the specificity of NP uptake by the same cells. This improved cellular specificity of PNBs was demonstrated in six different cell models representing diverse molecular targets such as epidermal growth factor receptor, CD3 receptor, prostate specific membrane antigen and mucin molecule MUC1. Thus PNBs may be a universal method and nano-agent that overcome the problem of non-specific uptake of NPs by non-target cells and improve the specificity of NP-based diagnostics, therapeutics and theranostics at the cell level.
UR - http://www.scopus.com/inward/record.url?scp=84859256793&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84859256793&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0034537
DO - 10.1371/journal.pone.0034537
M3 - Article
C2 - 22509318
AN - SCOPUS:84859256793
SN - 1932-6203
VL - 7
JO - PloS one
JF - PloS one
IS - 4
M1 - e34537
ER -