TY - JOUR
T1 - Improved Radiolabeling of PEGylated Protein
T2 - PEGylated Annexin V for Noninvasive Imaging of Tumor Apoptosis
AU - Wen, Xiaoxia
AU - Wu, Qing Ping
AU - Ke, Shi
AU - Wallace, Sidney
AU - Charnsangavej, Chusilp
AU - Huang, Peng
AU - Liang, Dong
AU - Chow, Diana
AU - Li, Chun
PY - 2003
Y1 - 2003
N2 - We have developed a one-step procedure to introduce both polyethylene glycol (PEG) and the metal chelator diethylenetriaminepentaacetic acid (DTPA) to proteins through a heterofunctional PEG precursor. The PEG precursor contains DTPA at one end and an amine-reactive isothiocyanate (SCN-) functional group at the other end. It was obtained as lyophilized powder and could be stored at 4°C for several months. Protein conjugation was achieved by simply mixing the proteins and the PEG precursor SCN-PEG-DTPA in an aqueous solution. As exemplified by the PEGylation and radiolabeling of annexin V, the resulting conjugate 111In-DTPA-PEG-annexin V showed selective binding to apoptotic cells in vitro and increased blood half-life in vivo. The PEGylated, radiolabeled annexin V may be useful in the non-invasive imaging of apoptosis.
AB - We have developed a one-step procedure to introduce both polyethylene glycol (PEG) and the metal chelator diethylenetriaminepentaacetic acid (DTPA) to proteins through a heterofunctional PEG precursor. The PEG precursor contains DTPA at one end and an amine-reactive isothiocyanate (SCN-) functional group at the other end. It was obtained as lyophilized powder and could be stored at 4°C for several months. Protein conjugation was achieved by simply mixing the proteins and the PEG precursor SCN-PEG-DTPA in an aqueous solution. As exemplified by the PEGylation and radiolabeling of annexin V, the resulting conjugate 111In-DTPA-PEG-annexin V showed selective binding to apoptotic cells in vitro and increased blood half-life in vivo. The PEGylated, radiolabeled annexin V may be useful in the non-invasive imaging of apoptosis.
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U2 - 10.1089/108497803770418364
DO - 10.1089/108497803770418364
M3 - Article
C2 - 14629830
AN - SCOPUS:0242665459
SN - 1084-9785
VL - 18
SP - 819
EP - 827
JO - Cancer Biotherapy and Radiopharmaceuticals
JF - Cancer Biotherapy and Radiopharmaceuticals
IS - 5
ER -