In vitro selection of DNA aptamers to glioblastoma multiforme

Abdullah Tahir Bayrac; Kwame Sefah; Parag Parekh; Ceren Bayrac; Basri Gulbakan; Huseyin Avni Oktem; Weihong Tan

doi:10.1021/cn100114k

In vitro selection of DNA aptamers to glioblastoma multiforme

Abdullah Tahir Bayrac, Kwame Sefah, Parag Parekh, Ceren Bayrac, Basri Gulbakan, Huseyin Avni Oktem, Weihong Tan

Endocrine Neoplasia & HD

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95 Scopus citations

Abstract

Aptamer probes for specific recognition of glioblastoma multiforme were generated using a repetitive and broad cell-SELEX-based procedure without negative selection. The 454 sequencing technology was used to monitor SELEX, and bioinformatics tools were used to identify aptamers from high throughput data. A group of aptamers were generated that can bind to target cells specifically with dissociation constants (K_d) in the nanomolar range. Selected aptamers showed high affinity to different types of glioblastoma cell lines while showing little or no affinity to other cancer cell lines. The aptamers generated in this study have potential use in different applications, such as probes for diagnosis and devices for targeted drug delivery, as well as tools for molecular marker discovery for glioblastomas.

Original language	English (US)
Pages (from-to)	175-181
Number of pages	7
Journal	ACS Chemical Neuroscience
Volume	2
Issue number	3
DOIs	https://doi.org/10.1021/cn100114k
State	Published - Mar 16 2011

Keywords

Cell-SELEX
DNA aptamer
cancer
glioblastoma

ASJC Scopus subject areas

Biochemistry
Physiology
Cognitive Neuroscience
Cell Biology

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10.1021/cn100114k

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AU - Bayrac, Abdullah Tahir

AU - Sefah, Kwame

AU - Parekh, Parag

AU - Bayrac, Ceren

AU - Gulbakan, Basri

AU - Oktem, Huseyin Avni

AU - Tan, Weihong

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In vitro selection of DNA aptamers to glioblastoma multiforme

Abstract

Keywords

ASJC Scopus subject areas

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