TY - JOUR
T1 - in vitro thermo‐chemosensitivity screening of spontaneous human tumors
T2 - Significant potentiation for cisplatin but not adriamycin
AU - Calabro, Annamaria
AU - Eva Singletary, S.
AU - Tucker, Susan
AU - Boddie, Arthur
AU - Spitzer, Gary
AU - Cavaliere, Renato
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1989/3/15
Y1 - 1989/3/15
N2 - The in vitro thermal enhancement of Adriamycin (ADR) and Cisplatin (CDDP) was investigated in 18 surgical biopsy specimens of human tumors cultured in the Adhesive Tumor Cell Culture System. Experimental conditions were adopted to simulate “therapeutic” trials: (a) temperature of 37.0°C, 40.5°C or 42.5°C; (b) hyperthermic duration of 30, 60, or 120 min; and (c) 4‐dose drug range normalized to human bone marrow toxicity. Drug concentrations that inhibited 90% of tumor growth (IC90) at 37.0°C were compared to the IC90 at 40.5°C and 42.5°C, adjusted for the effect of heat alone. CDDP plus heat was a better combination than ADR plus heat, regardless of the temperature and the exposure duration: significant synergism (p < 0.001) occurred in 37% of heat‐CDDP combinations, as compared with 15% of heat‐ADR combinations, and antagonism was significantly lower (p < 0.001) for heat‐CDDP than for heat‐ADR (4.4% versus 21% of combinations, respectively). Within the CDDP group, higher temperature and longer heat exposure resulted in an increased incidence of chemosensitivity. No specific pattern of synergism was evident within the ADR group, but a trend toward a higher incidence of antagonistic effects with increasing hyperthermic duration was observed.
AB - The in vitro thermal enhancement of Adriamycin (ADR) and Cisplatin (CDDP) was investigated in 18 surgical biopsy specimens of human tumors cultured in the Adhesive Tumor Cell Culture System. Experimental conditions were adopted to simulate “therapeutic” trials: (a) temperature of 37.0°C, 40.5°C or 42.5°C; (b) hyperthermic duration of 30, 60, or 120 min; and (c) 4‐dose drug range normalized to human bone marrow toxicity. Drug concentrations that inhibited 90% of tumor growth (IC90) at 37.0°C were compared to the IC90 at 40.5°C and 42.5°C, adjusted for the effect of heat alone. CDDP plus heat was a better combination than ADR plus heat, regardless of the temperature and the exposure duration: significant synergism (p < 0.001) occurred in 37% of heat‐CDDP combinations, as compared with 15% of heat‐ADR combinations, and antagonism was significantly lower (p < 0.001) for heat‐CDDP than for heat‐ADR (4.4% versus 21% of combinations, respectively). Within the CDDP group, higher temperature and longer heat exposure resulted in an increased incidence of chemosensitivity. No specific pattern of synergism was evident within the ADR group, but a trend toward a higher incidence of antagonistic effects with increasing hyperthermic duration was observed.
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U2 - 10.1002/ijc.2910430306
DO - 10.1002/ijc.2910430306
M3 - Article
C2 - 2925271
AN - SCOPUS:0024512586
SN - 0020-7136
VL - 43
SP - 385
EP - 390
JO - International journal of cancer
JF - International journal of cancer
IS - 3
ER -